Mechanical and morphological responses of osteoblast-like cells to two-photon polymerized microgrooved surfaces
© 2022 Wiley Periodicals LLC..
Microgrooved surfaces are recognized as an important strategy of tissue engineering to promote the alignment of bone cells. In this work, we have investigated the mechanical and morphological aspects of osteoblasts cells after interaction with different micro-structured polymeric surfaces. Femtosecond laser writing technique was used for the construction of circular and parallel microgrooved patterns in biocompatible polymeric surfaces based on pentaerythritol triacrylate. Additionally, we have studied the influence of the biocompatible TiO2 nanocrystals (NCs) related to the cell behavior, when incorporated to the photoresin. The atomic force microscopy technique was used to investigate the biomechanical reaction of the human osteoblast-like MG-63 cells for the different microgroove. It was demonstrated that osteoblasts grown on circular microgrooved surfaces exhibited significantly larger Young's modulus compared to cells sown on flat films. Furthermore, we could observe that TiO2 NCs improved the circular microgrooves effects, resulting in more populated sites, 34% more elongated cells, and increasing the cell stiffness by almost 160%. These results can guide the design and construction of effective scaffold surfaces with circular microgrooves for tissue engineering and bone regeneration.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:111 |
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| Enthalten in: |
Journal of biomedical materials research. Part A - 111(2023), 2 vom: 03. Feb., Seite 234-244 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dos Santos, Laura M S [VerfasserIn] |
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| Links: |
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| Themen: |
Cell adhesion |
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| Anmerkungen: |
Date Completed 15.12.2022 Date Revised 09.01.2023 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1002/jbm.a.37454 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM347541941 |
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| 100 | 1 | |a Dos Santos, Laura M S |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Mechanical and morphological responses of osteoblast-like cells to two-photon polymerized microgrooved surfaces |
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| 500 | |a Date Revised 09.01.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2022 Wiley Periodicals LLC. | ||
| 520 | |a Microgrooved surfaces are recognized as an important strategy of tissue engineering to promote the alignment of bone cells. In this work, we have investigated the mechanical and morphological aspects of osteoblasts cells after interaction with different micro-structured polymeric surfaces. Femtosecond laser writing technique was used for the construction of circular and parallel microgrooved patterns in biocompatible polymeric surfaces based on pentaerythritol triacrylate. Additionally, we have studied the influence of the biocompatible TiO2 nanocrystals (NCs) related to the cell behavior, when incorporated to the photoresin. The atomic force microscopy technique was used to investigate the biomechanical reaction of the human osteoblast-like MG-63 cells for the different microgroove. It was demonstrated that osteoblasts grown on circular microgrooved surfaces exhibited significantly larger Young's modulus compared to cells sown on flat films. Furthermore, we could observe that TiO2 NCs improved the circular microgrooves effects, resulting in more populated sites, 34% more elongated cells, and increasing the cell stiffness by almost 160%. These results can guide the design and construction of effective scaffold surfaces with circular microgrooves for tissue engineering and bone regeneration | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a cell adhesion | |
| 650 | 4 | |a circular microgrooves | |
| 650 | 4 | |a titanium dioxide nanocrystals | |
| 650 | 4 | |a two-photon polymerization | |
| 700 | 1 | |a de Oliveira, Jonathas M |e verfasserin |4 aut | |
| 700 | 1 | |a da Silva, Elaine C O |e verfasserin |4 aut | |
| 700 | 1 | |a Fonseca, Vitor M L |e verfasserin |4 aut | |
| 700 | 1 | |a Silva, Juliane P |e verfasserin |4 aut | |
| 700 | 1 | |a Barreto, Emiliano |e verfasserin |4 aut | |
| 700 | 1 | |a Dantas, Noelio Oliveira |e verfasserin |4 aut | |
| 700 | 1 | |a Silva, Anielle C A |e verfasserin |4 aut | |
| 700 | 1 | |a Jesus-Silva, Alcenísio J |e verfasserin |4 aut | |
| 700 | 1 | |a Mendonça, Cléber R |e verfasserin |4 aut | |
| 700 | 1 | |a Fonseca, Eduardo J S |e verfasserin |4 aut | |
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