Importance of surface charge of soot nanoparticles in determining inhalation toxicity in mice
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature..
Physicochemical properties of nanoparticles are important in regulating nanoparticle toxicity; however, the contribution of nanoparticle charge remains unclear. The objective of this study was to investigate the pulmonary effects of inhalation of charged soot nanoparticles. We established a stably charged nanoparticle generation system for whole-body exposure in BALB/c mice, which produced positively charged, negatively charged, and neutral soot nanoparticles in a wide range of concentrations. After a 7-day exposure, pulmonary toxicity was assessed, together with proteomics analysis. The charged soot nanoparticles on average carried 1.17-1.35 electric charges, and the sizes for nanoparticles under different charging conditions were all fixed at 69 ~ 72 nm. We observed that charged soot nanoparticles induced cytotoxic LDH and increased lung permeability, with the release of 8-isoprostane and caspase-3 and systemic IL-6 in mice, especially for positively charged soot nanoparticles. Next, we observed that positive-charged soot nanoparticles upregulated Eif2, Eif4, sirtuin, mammalian target of rapamycin (mTOR), peroxisome proliferator-activated receptors (PPAR), and HIPPO-related signaling pathways in the lungs compared with negatively charged soot nanoparticles. HIF1α, sirt1, E-cadherin, and Yap were increased in mice's lungs by positively charged soot nanoparticle exposure. In conclusion, carbonaceous nanoparticles carrying electric ions, especially positive-charged, are particularly toxic when inhaled and should be of concern in terms of pulmonary health protection.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
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| Enthalten in: |
Environmental science and pollution research international - 30(2023), 7 vom: 12. Feb., Seite 18985-18997 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hsiao, Ta-Chih [VerfasserIn] |
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| Links: |
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| Themen: |
Inflammation |
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| Anmerkungen: |
Date Completed 16.02.2023 Date Revised 16.02.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s11356-022-23444-4 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Physicochemical properties of nanoparticles are important in regulating nanoparticle toxicity; however, the contribution of nanoparticle charge remains unclear. The objective of this study was to investigate the pulmonary effects of inhalation of charged soot nanoparticles. We established a stably charged nanoparticle generation system for whole-body exposure in BALB/c mice, which produced positively charged, negatively charged, and neutral soot nanoparticles in a wide range of concentrations. After a 7-day exposure, pulmonary toxicity was assessed, together with proteomics analysis. The charged soot nanoparticles on average carried 1.17-1.35 electric charges, and the sizes for nanoparticles under different charging conditions were all fixed at 69 ~ 72 nm. We observed that charged soot nanoparticles induced cytotoxic LDH and increased lung permeability, with the release of 8-isoprostane and caspase-3 and systemic IL-6 in mice, especially for positively charged soot nanoparticles. Next, we observed that positive-charged soot nanoparticles upregulated Eif2, Eif4, sirtuin, mammalian target of rapamycin (mTOR), peroxisome proliferator-activated receptors (PPAR), and HIPPO-related signaling pathways in the lungs compared with negatively charged soot nanoparticles. HIF1α, sirt1, E-cadherin, and Yap were increased in mice's lungs by positively charged soot nanoparticle exposure. In conclusion, carbonaceous nanoparticles carrying electric ions, especially positive-charged, are particularly toxic when inhaled and should be of concern in terms of pulmonary health protection | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Inflammation | |
| 650 | 4 | |a Nanoparticle charge | |
| 650 | 4 | |a Nanoparticles | |
| 650 | 4 | |a Oxidative stress | |
| 650 | 4 | |a Particulate matter | |
| 650 | 4 | |a Polycyclic aromatic hydrocarbons | |
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| 700 | 1 | |a Yang, Tzu-Ting |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Yueh-Lun |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Yu-Fang |e verfasserin |4 aut | |
| 700 | 1 | |a Jheng, Yu-Teng |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Chii-Hong |e verfasserin |4 aut | |
| 700 | 1 | |a Chang, Jer-Hwa |e verfasserin |4 aut | |
| 700 | 1 | |a Chung, Kian Fan |e verfasserin |4 aut | |
| 700 | 1 | |a Kuo, Han-Pin |e verfasserin |4 aut | |
| 700 | 1 | |a Chuang, Hsiao-Chi |e verfasserin |4 aut | |
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