Complement and endothelial cell activation in COVID-19 patients compared to controls with suspected SARS-CoV-2 infection : A prospective cohort study
Copyright © 2022 Bruni, Charitos, Lampart, Moser, Siegemund, Bingisser, Osswald, Bassetti, Twerenbold, Trendelenburg, Rentsch and Osthoff..
Background: Thromboinflammation may influence disease outcome in COVID-19. We aimed to evaluate complement and endothelial cell activation in patients with confirmed COVID-19 compared to controls with clinically suspected but excluded SARS-CoV-2 infection.
Methods: In a prospective, observational, single-center study, patients presenting with clinically suspected COVID-19 were recruited in the emergency department. Blood samples on presentation were obtained for analysis of C5a, sC5b-9, E-selectin, Galectin-3, ICAM-1 and VCAM-1.
Results: 153 cases and 166 controls (suffering mainly from non-SARS-CoV-2 respiratory viral infections, non-infectious inflammatory conditions and bacterial pneumonia) were included. Hospital admission occurred in 62% and 45% of cases and controls, respectively. C5a and VCAM-1 concentrations were significantly elevated and E-selectin concentrations decreased in COVID-19 out- and inpatients compared to the respective controls. However, relative differences in outpatients vs. inpatients in most biomarkers were comparable between cases and controls. Elevated concentrations of C5a, Galectin-3, ICAM-1 and VCAM-1 on presentation were associated with the composite outcome of ICU- admission or 30-day mortality in COVID-19 and controls, yet more pronounced in COVID-19. C5a and sC5b-9 concentrations were significantly higher in COVID-19 males vs. females, which was not observed in the control group.
Conclusions: Our data indicate an activation of the complement cascade and endothelium in COVID-19 beyond a nonspecific inflammatory trigger as observed in controls (i.e., "over"-activation).
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2022 |
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Erschienen: |
2022 |
Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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Enthalten in: |
Frontiers in immunology - 13(2022) vom: 25., Seite 941742 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Bruni, Flavio [VerfasserIn] |
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Links: |
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Anmerkungen: |
Date Completed 10.10.2022 Date Revised 12.10.2022 published: Electronic-eCollection Citation Status MEDLINE |
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doi: |
10.3389/fimmu.2022.941742 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM347189512 |
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520 | |a Copyright © 2022 Bruni, Charitos, Lampart, Moser, Siegemund, Bingisser, Osswald, Bassetti, Twerenbold, Trendelenburg, Rentsch and Osthoff. | ||
520 | |a Background: Thromboinflammation may influence disease outcome in COVID-19. We aimed to evaluate complement and endothelial cell activation in patients with confirmed COVID-19 compared to controls with clinically suspected but excluded SARS-CoV-2 infection | ||
520 | |a Methods: In a prospective, observational, single-center study, patients presenting with clinically suspected COVID-19 were recruited in the emergency department. Blood samples on presentation were obtained for analysis of C5a, sC5b-9, E-selectin, Galectin-3, ICAM-1 and VCAM-1 | ||
520 | |a Results: 153 cases and 166 controls (suffering mainly from non-SARS-CoV-2 respiratory viral infections, non-infectious inflammatory conditions and bacterial pneumonia) were included. Hospital admission occurred in 62% and 45% of cases and controls, respectively. C5a and VCAM-1 concentrations were significantly elevated and E-selectin concentrations decreased in COVID-19 out- and inpatients compared to the respective controls. However, relative differences in outpatients vs. inpatients in most biomarkers were comparable between cases and controls. Elevated concentrations of C5a, Galectin-3, ICAM-1 and VCAM-1 on presentation were associated with the composite outcome of ICU- admission or 30-day mortality in COVID-19 and controls, yet more pronounced in COVID-19. C5a and sC5b-9 concentrations were significantly higher in COVID-19 males vs. females, which was not observed in the control group | ||
520 | |a Conclusions: Our data indicate an activation of the complement cascade and endothelium in COVID-19 beyond a nonspecific inflammatory trigger as observed in controls (i.e., "over"-activation) | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a COVID-19 | |
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700 | 1 | |a Charitos, Panteleimon |e verfasserin |4 aut | |
700 | 1 | |a Lampart, Maurin |e verfasserin |4 aut | |
700 | 1 | |a Moser, Stephan |e verfasserin |4 aut | |
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700 | 1 | |a Bingisser, Roland |e verfasserin |4 aut | |
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700 | 1 | |a Rentsch, Katharina M |e verfasserin |4 aut | |
700 | 1 | |a Osthoff, Michael |e verfasserin |4 aut | |
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