Defects in the expression of colonic host defense factors associate with barrier dysfunction induced by a high-fat/high-cholesterol diet
© 2022 American Association for Anatomy..
The effect of Western diets in the gastrointestinal system is largely mediated by their ability to promote alterations in the immunity and physiology of the intestinal epithelium, and to affect the composition of the commensal microbiota. To investigate the response of the colonic epithelium to high-fat/high-cholesterol diets (HFHCDs), we evaluated the synthesis of host defense factors involved in the maintenance of the colonic homeostasis. C57BL/6 mice were fed an HFHCD for 3 weeks and their colons were evaluated for histopathology, gene expression, and microbiota composition. In addition, intestinal permeability and susceptibility to Citrobacter rodentium were also studied. HFHCD caused colonic hyperplasia, loss of goblet cells, thinning of the mucus layer, moderate changes in the composition of the intestinal microbiota, and an increase in intestinal permeability. Gene expression analyses revealed significant drops in the transcript levels of Muc1, Muc2, Agr2, Atoh1, Spdef, Ang4, Camp, Tff3, Dmbt1, Fcgbp, Saa3, and Retnlb. The goblet cell granules of HFHCD-fed mice were devoid of Relmβ and Tff3, indicating defective production of those two factors critical for intestinal epithelial defense and homeostasis. In correspondence with these defects, colonic bacteria were in close contact with, and invading the epithelium. Fecal shedding of C. rodentium showed an increased bacterial burden in HFHCD-fed animals accompanied by increased epithelial damage. Collectively, our results show that HFHCD perturbs the synthesis of colonic host defense factors, which associate with alterations in the commensal microbiota, the integrity of the intestinal barrier, and the host's susceptibility to enteric infections.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:306 |
|---|---|
| Enthalten in: |
Anatomical record (Hoboken, N.J. : 2007) - 306(2023), 5 vom: 28. Mai, Seite 1165-1183 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Valdes, Jennifer [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
C. rodentium colitis |
|---|
| Anmerkungen: |
Date Completed 14.04.2023 Date Revised 17.04.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1002/ar.25083 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM347123988 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM347123988 | ||
| 003 | DE-627 | ||
| 005 | 20231226033110.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/ar.25083 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1157.xml |
| 035 | |a (DE-627)NLM347123988 | ||
| 035 | |a (NLM)36196983 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Valdes, Jennifer |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Defects in the expression of colonic host defense factors associate with barrier dysfunction induced by a high-fat/high-cholesterol diet |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 14.04.2023 | ||
| 500 | |a Date Revised 17.04.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2022 American Association for Anatomy. | ||
| 520 | |a The effect of Western diets in the gastrointestinal system is largely mediated by their ability to promote alterations in the immunity and physiology of the intestinal epithelium, and to affect the composition of the commensal microbiota. To investigate the response of the colonic epithelium to high-fat/high-cholesterol diets (HFHCDs), we evaluated the synthesis of host defense factors involved in the maintenance of the colonic homeostasis. C57BL/6 mice were fed an HFHCD for 3 weeks and their colons were evaluated for histopathology, gene expression, and microbiota composition. In addition, intestinal permeability and susceptibility to Citrobacter rodentium were also studied. HFHCD caused colonic hyperplasia, loss of goblet cells, thinning of the mucus layer, moderate changes in the composition of the intestinal microbiota, and an increase in intestinal permeability. Gene expression analyses revealed significant drops in the transcript levels of Muc1, Muc2, Agr2, Atoh1, Spdef, Ang4, Camp, Tff3, Dmbt1, Fcgbp, Saa3, and Retnlb. The goblet cell granules of HFHCD-fed mice were devoid of Relmβ and Tff3, indicating defective production of those two factors critical for intestinal epithelial defense and homeostasis. In correspondence with these defects, colonic bacteria were in close contact with, and invading the epithelium. Fecal shedding of C. rodentium showed an increased bacterial burden in HFHCD-fed animals accompanied by increased epithelial damage. Collectively, our results show that HFHCD perturbs the synthesis of colonic host defense factors, which associate with alterations in the commensal microbiota, the integrity of the intestinal barrier, and the host's susceptibility to enteric infections | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a C. rodentium colitis | |
| 650 | 4 | |a colonic host defense peptides | |
| 650 | 4 | |a epithelial damage | |
| 650 | 4 | |a goblet cells | |
| 650 | 4 | |a high-fat/high cholesterol diets | |
| 650 | 4 | |a intestinal permeability | |
| 650 | 4 | |a mucus layer | |
| 700 | 1 | |a Gagné-Sansfaçon, Jessica |e verfasserin |4 aut | |
| 700 | 1 | |a Reyes, Vilcy |e verfasserin |4 aut | |
| 700 | 1 | |a Armas, Anny |e verfasserin |4 aut | |
| 700 | 1 | |a Marrero, Gisela |e verfasserin |4 aut | |
| 700 | 1 | |a Moyo-Muamba, Mitterrand |e verfasserin |4 aut | |
| 700 | 1 | |a Ramanathan, Sheela |e verfasserin |4 aut | |
| 700 | 1 | |a Perreault, Nathalie |e verfasserin |4 aut | |
| 700 | 1 | |a Ilangumaran, Subburaj |e verfasserin |4 aut | |
| 700 | 1 | |a Rivard, Nathalie |e verfasserin |4 aut | |
| 700 | 1 | |a Fortier, Louis-Charles |e verfasserin |4 aut | |
| 700 | 1 | |a Menendez, Alfredo |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Anatomical record (Hoboken, N.J. : 2007) |d 2007 |g 306(2023), 5 vom: 28. Mai, Seite 1165-1183 |w (DE-627)NLM168836823 |x 1932-8494 |7 nnns |
| 773 | 1 | 8 | |g volume:306 |g year:2023 |g number:5 |g day:28 |g month:05 |g pages:1165-1183 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/ar.25083 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 306 |j 2023 |e 5 |b 28 |c 05 |h 1165-1183 | ||