ANGEL2 phosphatase activity is required for non-canonical mitochondrial RNA processing
© 2022. The Author(s)..
Canonical RNA processing in mammalian mitochondria is defined by tRNAs acting as recognition sites for nucleases to release flanking transcripts. The relevant factors, their structures, and mechanism are well described, but not all mitochondrial transcripts are punctuated by tRNAs, and their mode of processing has remained unsolved. Using Drosophila and mouse models, we demonstrate that non-canonical processing results in the formation of 3' phosphates, and that phosphatase activity by the carbon catabolite repressor 4 domain-containing family member ANGEL2 is required for their hydrolysis. Furthermore, our data suggest that members of the FAST kinase domain-containing protein family are responsible for these 3' phosphates. Our results therefore propose a mechanism for non-canonical RNA processing in metazoan mitochondria, by identifying the role of ANGEL2.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Nature communications - 13(2022), 1 vom: 30. Sept., Seite 5750 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Clemente, Paula [VerfasserIn] |
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| Links: |
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| Themen: |
63231-63-0 |
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| Anmerkungen: |
Date Completed 04.10.2022 Date Revised 28.11.2022 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41467-022-33368-9 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM346959373 |
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| 520 | |a © 2022. The Author(s). | ||
| 520 | |a Canonical RNA processing in mammalian mitochondria is defined by tRNAs acting as recognition sites for nucleases to release flanking transcripts. The relevant factors, their structures, and mechanism are well described, but not all mitochondrial transcripts are punctuated by tRNAs, and their mode of processing has remained unsolved. Using Drosophila and mouse models, we demonstrate that non-canonical processing results in the formation of 3' phosphates, and that phosphatase activity by the carbon catabolite repressor 4 domain-containing family member ANGEL2 is required for their hydrolysis. Furthermore, our data suggest that members of the FAST kinase domain-containing protein family are responsible for these 3' phosphates. Our results therefore propose a mechanism for non-canonical RNA processing in metazoan mitochondria, by identifying the role of ANGEL2 | ||
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| 700 | 1 | |a Calvo-Garrido, Javier |e verfasserin |4 aut | |
| 700 | 1 | |a Pearce, Sarah F |e verfasserin |4 aut | |
| 700 | 1 | |a Schober, Florian A |e verfasserin |4 aut | |
| 700 | 1 | |a Shigematsu, Megumi |e verfasserin |4 aut | |
| 700 | 1 | |a Siira, Stefan J |e verfasserin |4 aut | |
| 700 | 1 | |a Laine, Isabelle |e verfasserin |4 aut | |
| 700 | 1 | |a Spåhr, Henrik |e verfasserin |4 aut | |
| 700 | 1 | |a Steinmetzger, Christian |e verfasserin |4 aut | |
| 700 | 1 | |a Petzold, Katja |e verfasserin |4 aut | |
| 700 | 1 | |a Kirino, Yohei |e verfasserin |4 aut | |
| 700 | 1 | |a Wibom, Rolf |e verfasserin |4 aut | |
| 700 | 1 | |a Rackham, Oliver |e verfasserin |4 aut | |
| 700 | 1 | |a Filipovska, Aleksandra |e verfasserin |4 aut | |
| 700 | 1 | |a Rorbach, Joanna |e verfasserin |4 aut | |
| 700 | 1 | |a Freyer, Christoph |e verfasserin |4 aut | |
| 700 | 1 | |a Wredenberg, Anna |e verfasserin |4 aut | |
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