A Split CRISPR-Cpf1 Platform for Inducible Gene Activation
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
The CRISPR-Cpf1 also known as Cas12a is an RNA-guided endonuclease similar to CRISPR-Cas9. Combining the CRISPR-Cpf1 with optogenetics technology, we have engineered photoactivatable Cpf1 (paCpf1) to precisely control the genome sequence in a spatiotemporal manner. We also identified spontaneously activated split Cpf1 and thereby developed a potent dCpf1 split activator, which has the potential to activate endogenous target genes. Here we describe a method for optogenetic endogenous genome editing using paCpf1 in mammalian cells. Furthermore, we show a method for endogenous gene activation using dCpf1 split activator in mammalian cells and mice.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2577 |
|---|---|
| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2577(2023) vom: 29., Seite 229-240 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Otabe, Takahiro [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 03.10.2022 Date Revised 11.10.2022 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1007/978-1-0716-2724-2_16 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM346891310 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM346891310 | ||
| 003 | DE-627 | ||
| 005 | 20231226032531.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/978-1-0716-2724-2_16 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1156.xml |
| 035 | |a (DE-627)NLM346891310 | ||
| 035 | |a (NLM)36173577 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Otabe, Takahiro |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A Split CRISPR-Cpf1 Platform for Inducible Gene Activation |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 03.10.2022 | ||
| 500 | |a Date Revised 11.10.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. | ||
| 520 | |a The CRISPR-Cpf1 also known as Cas12a is an RNA-guided endonuclease similar to CRISPR-Cas9. Combining the CRISPR-Cpf1 with optogenetics technology, we have engineered photoactivatable Cpf1 (paCpf1) to precisely control the genome sequence in a spatiotemporal manner. We also identified spontaneously activated split Cpf1 and thereby developed a potent dCpf1 split activator, which has the potential to activate endogenous target genes. Here we describe a method for optogenetic endogenous genome editing using paCpf1 in mammalian cells. Furthermore, we show a method for endogenous gene activation using dCpf1 split activator in mammalian cells and mice | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a CRISPR–Cpf1 (also known as Cas12a) | |
| 650 | 4 | |a Gene activation | |
| 650 | 4 | |a Genome editing | |
| 650 | 4 | |a Genomic PCR | |
| 650 | 4 | |a Hydrodynamic tail vein injection | |
| 650 | 4 | |a In vivo gene activation | |
| 650 | 4 | |a NHEJ | |
| 650 | 4 | |a Optogenetics | |
| 650 | 4 | |a T7E1 | |
| 650 | 4 | |a crRNA | |
| 650 | 7 | |a RNA |2 NLM | |
| 650 | 7 | |a 63231-63-0 |2 NLM | |
| 650 | 7 | |a Endonucleases |2 NLM | |
| 650 | 7 | |a EC 3.1.- |2 NLM | |
| 700 | 1 | |a Nihongaki, Yuta |e verfasserin |4 aut | |
| 700 | 1 | |a Sato, Moritoshi |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Methods in molecular biology (Clifton, N.J.) |d 1984 |g 2577(2023) vom: 29., Seite 229-240 |w (DE-627)NLM074849794 |x 1940-6029 |7 nnns |
| 773 | 1 | 8 | |g volume:2577 |g year:2023 |g day:29 |g pages:229-240 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/978-1-0716-2724-2_16 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 2577 |j 2023 |b 29 |h 229-240 | ||