Capillary-Assisted Molecular Pendulum Bioanalysis
The development of robust biosensing strategies that can be easily implemented in everyday life remains a challenge for the future of modern biosensor research. While several reagentless approaches have attempted to address this challenge, they often achieve user-friendliness through sacrificing sensitivity or universality. While acceptable for certain applications, these trade-offs hinder the widespread adoption of reagentless biosensing technologies. Here, we report a novel approach to reagentless biosensing that achieves high sensitivity, rapid detection, and universality using the SARS-CoV-2 virus as a model target. Universality is achieved by using nanoscale molecular pendulums, which enables reagentless electrochemical biosensing through a variable antibody recognition element. Enhanced sensitivity and rapid detection are accomplished by incorporating the coffee-ring phenomenon into the sensing scheme, allowing for target preconcentration on a ring-shaped electrode. Using this approach, we obtained limits of detection of 1 fg/mL and 20 copies/mL for the SARS-CoV-2 nucleoproteins and viral particles, respectively. In addition, clinical sample analysis showed excellent agreement with Ct values from PCR-positive SARS-CoV-2 patients.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:144 |
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| Enthalten in: |
Journal of the American Chemical Society - 144(2022), 40 vom: 12. Okt., Seite 18338-18349 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zargartalebi, Hossein [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 13.10.2022 Date Revised 14.10.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/jacs.2c06192 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM346889359 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The development of robust biosensing strategies that can be easily implemented in everyday life remains a challenge for the future of modern biosensor research. While several reagentless approaches have attempted to address this challenge, they often achieve user-friendliness through sacrificing sensitivity or universality. While acceptable for certain applications, these trade-offs hinder the widespread adoption of reagentless biosensing technologies. Here, we report a novel approach to reagentless biosensing that achieves high sensitivity, rapid detection, and universality using the SARS-CoV-2 virus as a model target. Universality is achieved by using nanoscale molecular pendulums, which enables reagentless electrochemical biosensing through a variable antibody recognition element. Enhanced sensitivity and rapid detection are accomplished by incorporating the coffee-ring phenomenon into the sensing scheme, allowing for target preconcentration on a ring-shaped electrode. Using this approach, we obtained limits of detection of 1 fg/mL and 20 copies/mL for the SARS-CoV-2 nucleoproteins and viral particles, respectively. In addition, clinical sample analysis showed excellent agreement with Ct values from PCR-positive SARS-CoV-2 patients | ||
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| 700 | 1 | |a Flynn, Connor D |e verfasserin |4 aut | |
| 700 | 1 | |a Gomis, Surath |e verfasserin |4 aut | |
| 700 | 1 | |a Das, Jagotamoy |e verfasserin |4 aut | |
| 700 | 1 | |a Young, Tiana L |e verfasserin |4 aut | |
| 700 | 1 | |a Chien, Emily |e verfasserin |4 aut | |
| 700 | 1 | |a Mubareka, Samira |e verfasserin |4 aut | |
| 700 | 1 | |a McGeer, Allison |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Hansen |e verfasserin |4 aut | |
| 700 | 1 | |a Sargent, Edward H |e verfasserin |4 aut | |
| 700 | 1 | |a Nezhad, Amir Sanati |e verfasserin |4 aut | |
| 700 | 1 | |a Kelley, Shana O |e verfasserin |4 aut | |
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