Detection of biomarkers for filoviral infection with a silicon photonic resonator platform
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved..
This protocol describes the use of silicon photonic microring resonator sensors for detection of Ebola virus (EBOV) and Sudan virus (SUDV) soluble glycoprotein (sGP). This protocol encompasses biosensor functionalization of silicon microring resonator chips, detection of protein biomarkers in sera, preparing calibration standards for analytical validation, and quantification of the results from these experiments. This protocol is readily adaptable toward other analytes, including cytokines, chemokines, nucleic acids, and viruses. For complete details on the use and execution of this protocol, please refer to Qavi et al. (2022).
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:3 |
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| Enthalten in: |
STAR protocols - 3(2022), 4 vom: 16. Dez., Seite 101719 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Meserve, Krista [VerfasserIn] |
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| Links: |
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| Themen: |
Antibody |
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| Anmerkungen: |
Date Completed 21.12.2022 Date Revised 21.12.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.xpro.2022.101719 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a This protocol describes the use of silicon photonic microring resonator sensors for detection of Ebola virus (EBOV) and Sudan virus (SUDV) soluble glycoprotein (sGP). This protocol encompasses biosensor functionalization of silicon microring resonator chips, detection of protein biomarkers in sera, preparing calibration standards for analytical validation, and quantification of the results from these experiments. This protocol is readily adaptable toward other analytes, including cytokines, chemokines, nucleic acids, and viruses. For complete details on the use and execution of this protocol, please refer to Qavi et al. (2022) | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Biotechnology and bioengineering | |
| 650 | 4 | |a Chemistry | |
| 650 | 4 | |a Health sciences | |
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| 700 | 1 | |a Qavi, Abraham J |e verfasserin |4 aut | |
| 700 | 1 | |a Aman, M Javad |e verfasserin |4 aut | |
| 700 | 1 | |a Vu, Hong |e verfasserin |4 aut | |
| 700 | 1 | |a Zeitlin, Larry |e verfasserin |4 aut | |
| 700 | 1 | |a Dye, John M |e verfasserin |4 aut | |
| 700 | 1 | |a Froude, Jeffrey W |e verfasserin |4 aut | |
| 700 | 1 | |a Leung, Daisy W |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Lan |e verfasserin |4 aut | |
| 700 | 1 | |a Holtsberg, Frederick W |e verfasserin |4 aut | |
| 700 | 1 | |a Amarasinghe, Gaya K |e verfasserin |4 aut | |
| 700 | 1 | |a Bailey, Ryan C |e verfasserin |4 aut | |
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