Isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved..
Differences in metabolic profiles can link to functional changes of immune cells in disease conditions. Here, we detail a protocol for the detection and quantitation of 19 metabolites in one analytical run. We provide the parameters for chromatographic separation and mass spectrometric analysis of isotopically labeled and unlabeled metabolites. We include steps for incubation and sample preparation of PBMCs and monocytes. This protocol overcomes the chromatographic challenges caused by the chelating properties of some metabolites.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:3 |
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| Enthalten in: |
STAR protocols - 3(2022), 4 vom: 16. Dez., Seite 101715 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Giacomello, Ginevra [VerfasserIn] |
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| Links: |
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| Themen: |
Chemistry |
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| Anmerkungen: |
Date Completed 21.12.2022 Date Revised 25.01.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.xpro.2022.101715 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM346685869 |
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| 520 | |a Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Differences in metabolic profiles can link to functional changes of immune cells in disease conditions. Here, we detail a protocol for the detection and quantitation of 19 metabolites in one analytical run. We provide the parameters for chromatographic separation and mass spectrometric analysis of isotopically labeled and unlabeled metabolites. We include steps for incubation and sample preparation of PBMCs and monocytes. This protocol overcomes the chromatographic challenges caused by the chelating properties of some metabolites | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Chemistry | |
| 650 | 4 | |a Immunology | |
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