Immunofluorescence Labeling of Skeletal Muscle in Development, Regeneration, and Disease
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
Skeletal muscle is composed of long multinucleated cells, termed myofibers, that are formed through the activation and differentiation of resident muscle stem cells, called satellite cells. In healthy individuals, skeletal muscle enables voluntary locomotion while also playing a role in energy metabolism and thermoregulation. As skeletal muscle is integral to everyday processes, perturbations to skeletal muscle function can have devastating consequences. Here we describe an integral tool in biomedical research of skeletal muscle regeneration and disease, the immunofluorescence staining of myogenic cells. We highlight useful techniques for immunostaining myogenic cells, and we list validated antibodies for the staining of muscle proteins across different species and multiple developmental time points. This includes methods for unmasking antigens following formaldehyde fixation (using myosin heavy chain staining as an example) and practices for preserving endogenous fluorescent proteins by cardiac perfusion fixation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2566 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2566(2023) vom: 24., Seite 113-132 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Esper, Marie E [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 28.09.2022 Date Revised 24.05.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-2675-7_9 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM346685281 |
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| 520 | |a © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. | ||
| 520 | |a Skeletal muscle is composed of long multinucleated cells, termed myofibers, that are formed through the activation and differentiation of resident muscle stem cells, called satellite cells. In healthy individuals, skeletal muscle enables voluntary locomotion while also playing a role in energy metabolism and thermoregulation. As skeletal muscle is integral to everyday processes, perturbations to skeletal muscle function can have devastating consequences. Here we describe an integral tool in biomedical research of skeletal muscle regeneration and disease, the immunofluorescence staining of myogenic cells. We highlight useful techniques for immunostaining myogenic cells, and we list validated antibodies for the staining of muscle proteins across different species and multiple developmental time points. This includes methods for unmasking antigens following formaldehyde fixation (using myosin heavy chain staining as an example) and practices for preserving endogenous fluorescent proteins by cardiac perfusion fixation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Antibodies | |
| 650 | 4 | |a Differentiation | |
| 650 | 4 | |a Heat-induced antigen retrieval | |
| 650 | 4 | |a Immunofluorescence | |
| 650 | 4 | |a Immunohistochemistry | |
| 650 | 4 | |a Muscle stem cell | |
| 650 | 4 | |a Myogenesis | |
| 650 | 4 | |a Myosin heavy chain | |
| 650 | 4 | |a Pax7 | |
| 650 | 4 | |a Regeneration | |
| 650 | 4 | |a Satellite sell | |
| 650 | 4 | |a Skeletal muscle | |
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| 700 | 1 | |a Rudnicki, Michael A |e verfasserin |4 aut | |
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