Nanoscale laser-induced breakdown spectroscopy imaging reveals chemical distribution with subcellular resolution
This journal is © The Royal Society of Chemistry..
Understanding chemical compositions is one of the most important parts in exploring the microscopic world. As a simple method for elemental detection, laser-induced breakdown spectroscopy (LIBS) is widely used in materials, geological and life science fields. However, due to the long-existing limitation in spatial resolution, it is difficult for LIBS to play an analytical role in the field of micro-world. Herein, we first report a reliable nanoscale resolution LIBS imaging technique by introducing a sampling laser with a micro-lensed fiber. Through the emission enhancement using the double-pulse laser, we obtained the spectral signal from a sampling crater of less than 500 nanometers in diameter, and visualized the chemical distribution of the self-made grid sample, SIM chip and nano-particles in single cells. The relative limits of detection (RLODs) of In and absolute limits of detection (ALODs) of Al can reach 0.6% and 18.3 fg, respectively.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2 |
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| Enthalten in: |
Nanoscale advances - 2(2020), 9 vom: 16. Sept., Seite 3983-3990 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Meng, Yifan [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Revised 24.09.2022 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1039/d0na00380h |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM346492033 |
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| 520 | |a This journal is © The Royal Society of Chemistry. | ||
| 520 | |a Understanding chemical compositions is one of the most important parts in exploring the microscopic world. As a simple method for elemental detection, laser-induced breakdown spectroscopy (LIBS) is widely used in materials, geological and life science fields. However, due to the long-existing limitation in spatial resolution, it is difficult for LIBS to play an analytical role in the field of micro-world. Herein, we first report a reliable nanoscale resolution LIBS imaging technique by introducing a sampling laser with a micro-lensed fiber. Through the emission enhancement using the double-pulse laser, we obtained the spectral signal from a sampling crater of less than 500 nanometers in diameter, and visualized the chemical distribution of the self-made grid sample, SIM chip and nano-particles in single cells. The relative limits of detection (RLODs) of In and absolute limits of detection (ALODs) of Al can reach 0.6% and 18.3 fg, respectively | ||
| 650 | 4 | |a Journal Article | |
| 700 | 1 | |a Gao, Chaohong |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Zheng |e verfasserin |4 aut | |
| 700 | 1 | |a Hang, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Benli |e verfasserin |4 aut | |
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