Changes in the TCR repertoire of T-cell subsets during culture of cytokine-induced killer cells
© 2022 Federation of European Biochemical Societies..
Cytokine-induced killer (CIK) cells are a group of highly heterogeneous T cells expressing diverse T-cell antigen receptors (TCRs), but possessing wide major histocompatibility complex (MHC)-unrestricted antitumour activity, which have been used in the treatment of many tumours. However, the clonal diversity of different T-cell subsets and whether there is a certain clonal preference during CIK culture remains to be clarified. We found that the proportion of oligoclones seen in some subfamilies of freshly isolated T cells decreased in varying degrees after culturing. This indicates that the diversity of T-cell clones had been restored to a certain extent. The diversity differed among different T-cell subsets, as some TCR subfamily oligoclones mainly appeared in CD3+ CD56+ cells, which also indicates the heterogeneity of the CIK cell composition.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:596 |
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| Enthalten in: |
FEBS letters - 596(2022), 20 vom: 21. Okt., Seite 2696-2705 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liang, Guozheng [VerfasserIn] |
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| Links: |
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| Themen: |
CIK |
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| Anmerkungen: |
Date Completed 26.10.2022 Date Revised 26.10.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/1873-3468.14501 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM346460603 |
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| 520 | |a Cytokine-induced killer (CIK) cells are a group of highly heterogeneous T cells expressing diverse T-cell antigen receptors (TCRs), but possessing wide major histocompatibility complex (MHC)-unrestricted antitumour activity, which have been used in the treatment of many tumours. However, the clonal diversity of different T-cell subsets and whether there is a certain clonal preference during CIK culture remains to be clarified. We found that the proportion of oligoclones seen in some subfamilies of freshly isolated T cells decreased in varying degrees after culturing. This indicates that the diversity of T-cell clones had been restored to a certain extent. The diversity differed among different T-cell subsets, as some TCR subfamily oligoclones mainly appeared in CD3+ CD56+ cells, which also indicates the heterogeneity of the CIK cell composition | ||
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| 700 | 1 | |a Chen, Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Jicong |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Chuandong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jiawen |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Han |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Fenglin |e verfasserin |4 aut | |
| 700 | 1 | |a Tao, Changli |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Wenfeng |e verfasserin |4 aut | |
| 700 | 1 | |a Shao, Hongwei |e verfasserin |4 aut | |
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