Small RNA-Sequencing Library Preparation for the Halophilic Archaeon Haloferax volcanii
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
Posttranscriptional regulation actuated by small RNAs (sRNAs) plays essential roles in a wide variety of cellular processes, especially in stress responses and environmental signaling. Hundreds of sRNAs have recently been discovered in archaea using genome-wide approaches but the molecular mechanisms of only a few have been characterized experimentally. Here, we describe how to build sRNA sequencing libraries using size-selected total RNA in the model archaeon, Haloferax volcanii , to provide a tool to further characterize sRNAs in archaea.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2522 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2522(2022) vom: 20., Seite 243-254 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Gelsinger, Diego Rivera [VerfasserIn] |
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| Links: |
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| Themen: |
Haloarchaea |
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| Anmerkungen: |
Date Completed 23.09.2022 Date Revised 26.09.2022 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-2445-6_15 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Posttranscriptional regulation actuated by small RNAs (sRNAs) plays essential roles in a wide variety of cellular processes, especially in stress responses and environmental signaling. Hundreds of sRNAs have recently been discovered in archaea using genome-wide approaches but the molecular mechanisms of only a few have been characterized experimentally. Here, we describe how to build sRNA sequencing libraries using size-selected total RNA in the model archaeon, Haloferax volcanii , to provide a tool to further characterize sRNAs in archaea | ||
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