Plasmid encoding miRNA-200c delivered by CaCO3-based nanoparticles enhances rat alveolar bone formation
Aim: miRNAs have been shown to improve the restoration of craniofacial bone defects. This work aimed to enhance transfection efficiency and miR-200c-induced bone formation in alveolar bone defects via plasmid DNA encoding miR-200c delivery from CaCO3 nanoparticles. Materials & methods: The CaCO3/miR-200c delivery system was evaluated in vitro (microscopy, transfection efficiency, biocompatibility) and miR-200c-induced in vivo alveolar bone formation was assessed via micro-computed tomography and histology. Results: CaCO3 nanoparticles significantly enhanced the transfection of plasmid DNA encoding miR-200c without inflammatory effects and sustained miR-200c expression. CaCO3/miR-200c treatment in vivo significantly increased bone formation in rat alveolar bone defects. Conclusion: CaCO3 nanoparticles enhance miR-200c delivery to accelerate alveolar bone formation, thereby demonstrating the application of CaCO3/miR-200c to craniofacial bone defects.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:17 |
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| Enthalten in: |
Nanomedicine (London, England) - 17(2022), 19 vom: 06. Aug., Seite 1339-1354 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Remy, Matthew T [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 23.11.2022 Date Revised 02.08.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.2217/nnm-2022-0151 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM346415624 |
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| 100 | 1 | |a Remy, Matthew T |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Plasmid encoding miRNA-200c delivered by CaCO3-based nanoparticles enhances rat alveolar bone formation |
| 264 | 1 | |c 2022 | |
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| 500 | |a Date Completed 23.11.2022 | ||
| 500 | |a Date Revised 02.08.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Aim: miRNAs have been shown to improve the restoration of craniofacial bone defects. This work aimed to enhance transfection efficiency and miR-200c-induced bone formation in alveolar bone defects via plasmid DNA encoding miR-200c delivery from CaCO3 nanoparticles. Materials & methods: The CaCO3/miR-200c delivery system was evaluated in vitro (microscopy, transfection efficiency, biocompatibility) and miR-200c-induced in vivo alveolar bone formation was assessed via micro-computed tomography and histology. Results: CaCO3 nanoparticles significantly enhanced the transfection of plasmid DNA encoding miR-200c without inflammatory effects and sustained miR-200c expression. CaCO3/miR-200c treatment in vivo significantly increased bone formation in rat alveolar bone defects. Conclusion: CaCO3 nanoparticles enhance miR-200c delivery to accelerate alveolar bone formation, thereby demonstrating the application of CaCO3/miR-200c to craniofacial bone defects | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a CaCO3 nanoparticles | |
| 650 | 4 | |a HEPM cells | |
| 650 | 4 | |a alveolar bone formation | |
| 650 | 4 | |a in vivo animal models | |
| 650 | 4 | |a miRNAs | |
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| 700 | 1 | |a Ding, Qiong |e verfasserin |4 aut | |
| 700 | 1 | |a Krongbaramee, Tadkamol |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Jue |e verfasserin |4 aut | |
| 700 | 1 | |a Mora Mata, Andrés V |e verfasserin |4 aut | |
| 700 | 1 | |a Haes, Amanda J |e verfasserin |4 aut | |
| 700 | 1 | |a Amendt, Brad A |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Hongli |e verfasserin |4 aut | |
| 700 | 1 | |a Buchakjian, Marisa R |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, Liu |e verfasserin |4 aut | |
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