Identification of CD8+ T cell subsets that normalize in early-treated people living with HIV receiving antiretroviral therapy
© 2022. The Author(s)..
BACKGROUND: Although combined antiretroviral therapy (cART) has decreased the mortality associated with HIV infection, complete immune reconstitution is not achieved despite viral suppression. Alterations of CD8+ T cells and some of their subpopulations, such as interleukin (IL)-17-producing cells, are evidenced in treated individuals and are associated with systemic inflammation and adverse disease outcomes. We sought to evaluate if different CD8+ T cell subsets are differentially normalized during a clinical follow-up of people living with HIV (PLWH) receiving suppressive cART.
METHODS: We explored the changes in the frequencies, activation/exhaustion phenotypes (HLA-DR, CD38, PD-1, and TIM-3), and function (total and HIV-specific cells expressing CD107a, perforin, granzyme B, interferon [IFN]-γ and IL-17) of CD8+ T cells from early-treated PLWH receiving cART in a 1-year follow-up, using a multidimensional flow cytometry approach.
RESULTS: Despite continuous cART-induced viral suppression and recovery of CD4+ T cells, after a 1-year follow-up, the CD8+ T cell counts, CD4:CD8 ratio, PD-1 expression, and IL-17 production by CD8+ T cells exhibited incomplete normalization compared with seronegative controls. However, the proportion of CD8+ T cells with an exhausted phenotype (co-expressing PD-1 andTIM-3), and cells co-expressing cytotoxic molecules (Perforin and Granzyme B), reached normalization.
CONCLUSIONS: Although suppressive cART achieves normalization of CD4+ T cell counts, only particular subsets of CD8+ T cells are more rapidly normalized in PLWH receiving cART, which could be routinely used as biomarkers for therapy efficiency in these patients.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:19 |
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| Enthalten in: |
AIDS research and therapy - 19(2022), 1 vom: 14. Sept., Seite 42 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Perdomo-Celis, Federico [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 16.09.2022 Date Revised 10.12.2022 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1186/s12981-022-00465-0 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM346215145 |
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| 100 | 1 | |a Perdomo-Celis, Federico |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Identification of CD8+ T cell subsets that normalize in early-treated people living with HIV receiving antiretroviral therapy |
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| 500 | |a Date Revised 10.12.2022 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2022. The Author(s). | ||
| 520 | |a BACKGROUND: Although combined antiretroviral therapy (cART) has decreased the mortality associated with HIV infection, complete immune reconstitution is not achieved despite viral suppression. Alterations of CD8+ T cells and some of their subpopulations, such as interleukin (IL)-17-producing cells, are evidenced in treated individuals and are associated with systemic inflammation and adverse disease outcomes. We sought to evaluate if different CD8+ T cell subsets are differentially normalized during a clinical follow-up of people living with HIV (PLWH) receiving suppressive cART | ||
| 520 | |a METHODS: We explored the changes in the frequencies, activation/exhaustion phenotypes (HLA-DR, CD38, PD-1, and TIM-3), and function (total and HIV-specific cells expressing CD107a, perforin, granzyme B, interferon [IFN]-γ and IL-17) of CD8+ T cells from early-treated PLWH receiving cART in a 1-year follow-up, using a multidimensional flow cytometry approach | ||
| 520 | |a RESULTS: Despite continuous cART-induced viral suppression and recovery of CD4+ T cells, after a 1-year follow-up, the CD8+ T cell counts, CD4:CD8 ratio, PD-1 expression, and IL-17 production by CD8+ T cells exhibited incomplete normalization compared with seronegative controls. However, the proportion of CD8+ T cells with an exhausted phenotype (co-expressing PD-1 andTIM-3), and cells co-expressing cytotoxic molecules (Perforin and Granzyme B), reached normalization | ||
| 520 | |a CONCLUSIONS: Although suppressive cART achieves normalization of CD4+ T cell counts, only particular subsets of CD8+ T cells are more rapidly normalized in PLWH receiving cART, which could be routinely used as biomarkers for therapy efficiency in these patients | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Antiretroviral therapy | |
| 650 | 4 | |a CD8-positive T-lymphocytes | |
| 650 | 4 | |a HIV | |
| 650 | 4 | |a Immune reconstitution | |
| 650 | 7 | |a Interleukin-17 |2 NLM | |
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| 700 | 1 | |a Arcia-Anaya, David |e verfasserin |4 aut | |
| 700 | 1 | |a Alzate, Juan Carlos |e verfasserin |4 aut | |
| 700 | 1 | |a Velilla, Paula A |e verfasserin |4 aut | |
| 700 | 1 | |a Díaz, Francisco J |e verfasserin |4 aut | |
| 700 | 1 | |a Posada, Maria Paulina |e verfasserin |4 aut | |
| 700 | 1 | |a Rugeles, María T |e verfasserin |4 aut | |
| 700 | 1 | |a Taborda, Natalia A |e verfasserin |4 aut | |
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