Comparative Evaluation of Different Surface Coatings of Fe3O4-Based Magnetic Nano Sorbent for Applications in the Nucleic Acids Extraction
Nucleic acid extraction and purification are crucial steps in sample preparation for multiple diagnostic procedures. Routine methodologies of DNA isolation require benchtop equipment (e.g., centrifuges) and labor-intensive steps. Magnetic nanoparticles (MNPs) as solid-phase sorbents could simplify this procedure. A wide range of surface coatings employs various molecular interactions between dsDNA and magnetic nano-sorbents. However, a reliable, comparative evaluation of their performance is complex. In this work, selected Fe3O4 modifications, i.e., polyethyleneimine, gold, silica, and graphene derivatives, were comprehensively evaluated for applications in dsDNA extraction. A family of single batch nanoparticles was compared in terms of morphology (STEM), composition (ICP-MS/MS and elemental analysis), surface coating (UV-Vis, TGA, FTIR), and MNP charge (ζ-potential). ICP-MS/MS was also used to unify MNPs concentration allowing a reliable assessment of individual coatings on DNA extraction. Moreover, studies on adsorption medium (monovalent vs. divalent ions) and extraction buffer composition were carried out. As a result, essential relationships between nanoparticle coatings and DNA adsorption efficiencies have been noticed. Fe3O4PEI MNPs turned out to be the most efficient nano sorbents. The optimized composition of the extraction buffer (medium containing 0.1 mM EDTA) helped avoid problems with Fe3+ stripping, which improved the validity of the spectroscopic determination of DNA recovery.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:23 |
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| Enthalten in: |
International journal of molecular sciences - 23(2022), 16 vom: 09. Aug. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Szymczyk, Anna [VerfasserIn] |
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| Links: |
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| Themen: |
DNA—nanoparticle interactions |
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| Anmerkungen: |
Date Completed 29.08.2022 Date Revised 30.08.2022 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/ijms23168860 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM345305337 |
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| 520 | |a Nucleic acid extraction and purification are crucial steps in sample preparation for multiple diagnostic procedures. Routine methodologies of DNA isolation require benchtop equipment (e.g., centrifuges) and labor-intensive steps. Magnetic nanoparticles (MNPs) as solid-phase sorbents could simplify this procedure. A wide range of surface coatings employs various molecular interactions between dsDNA and magnetic nano-sorbents. However, a reliable, comparative evaluation of their performance is complex. In this work, selected Fe3O4 modifications, i.e., polyethyleneimine, gold, silica, and graphene derivatives, were comprehensively evaluated for applications in dsDNA extraction. A family of single batch nanoparticles was compared in terms of morphology (STEM), composition (ICP-MS/MS and elemental analysis), surface coating (UV-Vis, TGA, FTIR), and MNP charge (ζ-potential). ICP-MS/MS was also used to unify MNPs concentration allowing a reliable assessment of individual coatings on DNA extraction. Moreover, studies on adsorption medium (monovalent vs. divalent ions) and extraction buffer composition were carried out. As a result, essential relationships between nanoparticle coatings and DNA adsorption efficiencies have been noticed. Fe3O4PEI MNPs turned out to be the most efficient nano sorbents. The optimized composition of the extraction buffer (medium containing 0.1 mM EDTA) helped avoid problems with Fe3+ stripping, which improved the validity of the spectroscopic determination of DNA recovery | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a DNA extraction | |
| 650 | 4 | |a DNA—nanoparticle interactions | |
| 650 | 4 | |a Fe3O4 MNPs functionalization | |
| 650 | 4 | |a iron(II,III) oxide | |
| 650 | 4 | |a magnetic nanoparticles synthesis | |
| 650 | 7 | |a Magnetite Nanoparticles |2 NLM | |
| 650 | 7 | |a Nucleic Acids |2 NLM | |
| 700 | 1 | |a Drozd, Marcin |e verfasserin |4 aut | |
| 700 | 1 | |a Kamińska, Agnieszka |e verfasserin |4 aut | |
| 700 | 1 | |a Matczuk, Magdalena |e verfasserin |4 aut | |
| 700 | 1 | |a Trzaskowski, Maciej |e verfasserin |4 aut | |
| 700 | 1 | |a Mazurkiewicz-Pawlicka, Marta |e verfasserin |4 aut | |
| 700 | 1 | |a Ziółkowski, Robert |e verfasserin |4 aut | |
| 700 | 1 | |a Malinowska, Elżbieta |e verfasserin |4 aut | |
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