Genetic disruption of nucleoside transporter 4 reveals its critical roles in malaria parasite sporozoite functions
All protozoan parasites are lacking the pathway to synthesize purines de novo and therefore they depend on their host cells to provide purines. A number of highly conserved nucleoside transporter (NT) proteins are encoded in malaria parasite genomes, of which NT1 is characterized in Plasmodium falciparum and P. yoelii as a plasma membrane protein that is responsible for salvage of purines from the host, and NT2 is an endoplasmic membrane NT protein. Whereas NT3 is only present in primate malaria parasites, little is known about NT4, which is conserved in all malaria parasite species. Herein, we targeted NT4 gene for deletion in P. berghei. NT4 knockout parasites developed normally as blood stages, ookinetes and formed oocysts with sporozoites compared with wild-type (WT) P. berghei ANKA parasites. However, nt4(-) sporozoites showed significantly decreased egress from oocysts to hemolymph, significant reduction of colonization of the salivary glands, and complete abolishment of infection of the mammalian host by salivary gland and hemolymph sporozoites. Therefore, we identify NT4 as a NT that is important, not for replication and growth, but for sporozoite infectivity functions.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:117 |
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| Enthalten in: |
Pathogens and global health - 117(2023), 3 vom: 21. Mai, Seite 284-292 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Deveci, Gozde [VerfasserIn] |
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| Links: |
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| Themen: |
Hemolymph sporozoites |
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| Anmerkungen: |
Date Completed 10.04.2023 Date Revised 25.08.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1080/20477724.2022.2112880 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM345215028 |
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| 245 | 1 | 0 | |a Genetic disruption of nucleoside transporter 4 reveals its critical roles in malaria parasite sporozoite functions |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a All protozoan parasites are lacking the pathway to synthesize purines de novo and therefore they depend on their host cells to provide purines. A number of highly conserved nucleoside transporter (NT) proteins are encoded in malaria parasite genomes, of which NT1 is characterized in Plasmodium falciparum and P. yoelii as a plasma membrane protein that is responsible for salvage of purines from the host, and NT2 is an endoplasmic membrane NT protein. Whereas NT3 is only present in primate malaria parasites, little is known about NT4, which is conserved in all malaria parasite species. Herein, we targeted NT4 gene for deletion in P. berghei. NT4 knockout parasites developed normally as blood stages, ookinetes and formed oocysts with sporozoites compared with wild-type (WT) P. berghei ANKA parasites. However, nt4(-) sporozoites showed significantly decreased egress from oocysts to hemolymph, significant reduction of colonization of the salivary glands, and complete abolishment of infection of the mammalian host by salivary gland and hemolymph sporozoites. Therefore, we identify NT4 as a NT that is important, not for replication and growth, but for sporozoite infectivity functions | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Malaria | |
| 650 | 4 | |a P. berghei ANKA | |
| 650 | 4 | |a hemolymph sporozoites | |
| 650 | 4 | |a nucleoside transporter 4 | |
| 650 | 4 | |a oocyst sporozoites | |
| 650 | 4 | |a salivary gland sporozoites | |
| 650 | 7 | |a Protozoan Proteins |2 NLM | |
| 700 | 1 | |a Kamil, Mohd |e verfasserin |4 aut | |
| 700 | 1 | |a Kina, Umit |e verfasserin |4 aut | |
| 700 | 1 | |a Temel, Binnur Aydogan |e verfasserin |4 aut | |
| 700 | 1 | |a Aly, Ahmed S I |e verfasserin |4 aut | |
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