Application value of blood metagenomic next-generation sequencing in patients with connective tissue diseases
Copyright © 2022 Su, Yan, Li, Ding, Li, Xie, Gao, Li and Wang..
Objective: This study aimed to analyze the application value of blood metagenomic next-generation sequencing (mNGS) in patients with connective tissue diseases (CTDs) to provide a reference for infection diagnosis and guidance for treatment.
Methods: A total of 126 CTD patients with suspected infections who were hospitalized in the Department of Rheumatology, the Second Hospital of Shanxi Medical University from January 2020 to December 2021 were enrolled in this study. We retrospectively reviewed the results of mNGS and conventional diagnostic tests (CDTs).
Results: Systemic lupus erythematosus (SLE) and polymyositis/dermatomyositis (DM/PM) had the highest incidence of infections. The positive pathogen detection rates of mNGS were higher than those of CDT. The virus infections are the most common type in CTD patients with single or mixed infection, especially Human gammaherpesvirus 4 (EBV), Human betaherpesvirus 5 (CMV), and Human alphaherpesvirus 1. The incidence of prokaryote and eukaryote infections is secondary to viruses. Bloodstream infections of rare pathogens such as Pneumocystis jirovecii should be of concern. Meanwhile, the most common mixed infection was bacterial-virus coinfection.
Conclusion: mNGS has incremental application value in patients with CTD suspected of co-infection. It has a high sensitivity, and a wide detection range for microorganisms in CTD patients. Furthermore, the high incidence of opportunistic virus infections in CTD patients should be of sufficient concern.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Frontiers in immunology - 13(2022) vom: 25., Seite 939057 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Su, Rui [VerfasserIn] |
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| Links: |
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| Themen: |
CMV (citomegalovirus) |
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| Anmerkungen: |
Date Completed 19.08.2022 Date Revised 22.08.2022 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.3389/fimmu.2022.939057 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM344979601 |
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| 500 | |a Date Revised 22.08.2022 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2022 Su, Yan, Li, Ding, Li, Xie, Gao, Li and Wang. | ||
| 520 | |a Objective: This study aimed to analyze the application value of blood metagenomic next-generation sequencing (mNGS) in patients with connective tissue diseases (CTDs) to provide a reference for infection diagnosis and guidance for treatment | ||
| 520 | |a Methods: A total of 126 CTD patients with suspected infections who were hospitalized in the Department of Rheumatology, the Second Hospital of Shanxi Medical University from January 2020 to December 2021 were enrolled in this study. We retrospectively reviewed the results of mNGS and conventional diagnostic tests (CDTs) | ||
| 520 | |a Results: Systemic lupus erythematosus (SLE) and polymyositis/dermatomyositis (DM/PM) had the highest incidence of infections. The positive pathogen detection rates of mNGS were higher than those of CDT. The virus infections are the most common type in CTD patients with single or mixed infection, especially Human gammaherpesvirus 4 (EBV), Human betaherpesvirus 5 (CMV), and Human alphaherpesvirus 1. The incidence of prokaryote and eukaryote infections is secondary to viruses. Bloodstream infections of rare pathogens such as Pneumocystis jirovecii should be of concern. Meanwhile, the most common mixed infection was bacterial-virus coinfection | ||
| 520 | |a Conclusion: mNGS has incremental application value in patients with CTD suspected of co-infection. It has a high sensitivity, and a wide detection range for microorganisms in CTD patients. Furthermore, the high incidence of opportunistic virus infections in CTD patients should be of sufficient concern | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a CMV (citomegalovirus) | |
| 650 | 4 | |a EBV - Epstein-Barr Virus | |
| 650 | 4 | |a connective tissue diseases | |
| 650 | 4 | |a infection | |
| 650 | 4 | |a metagenomic next-generation sequencing | |
| 650 | 4 | |a virus | |
| 700 | 1 | |a Yan, Huanhuan |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Na |e verfasserin |4 aut | |
| 700 | 1 | |a Ding, Tingting |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Baochen |e verfasserin |4 aut | |
| 700 | 1 | |a Xie, Yuhuan |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Chong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xiaofeng |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Caihong |e verfasserin |4 aut | |
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