Standardization of Synthetic Biology Tools and Assembly Methods for Saccharomyces cerevisiae and Emerging Yeast Species
As redesigning organisms using engineering principles is one of the purposes of synthetic biology (SynBio), the standardization of experimental methods and DNA parts is becoming increasingly a necessity. The synthetic biology community focusing on the engineering of Saccharomyces cerevisiae has been in the foreground in this area, conceiving several well-characterized SynBio toolkits widely adopted by the community. In this review, the molecular methods and toolkits developed for S. cerevisiae are discussed in terms of their contributions to the required standardization efforts. In addition, the toolkits designed for emerging nonconventional yeast species including Yarrowia lipolytica, Komagataella phaffii, and Kluyveromyces marxianus are also reviewed. Without a doubt, the characterized DNA parts combined with the standardized assembly strategies highlighted in these toolkits have greatly contributed to the rapid development of many metabolic engineering and diagnostics applications among others. Despite the growing capacity in deploying synthetic biology for common yeast genome engineering works, the yeast community has a long journey to go to exploit it in more sophisticated and delicate applications like bioautomation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
ACS synthetic biology - 11(2022), 8 vom: 19. Aug., Seite 2527-2547 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Malcı, Koray [VerfasserIn] |
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| Links: |
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| Themen: |
Automation |
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| Anmerkungen: |
Date Completed 22.08.2022 Date Revised 08.12.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/acssynbio.1c00442 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a As redesigning organisms using engineering principles is one of the purposes of synthetic biology (SynBio), the standardization of experimental methods and DNA parts is becoming increasingly a necessity. The synthetic biology community focusing on the engineering of Saccharomyces cerevisiae has been in the foreground in this area, conceiving several well-characterized SynBio toolkits widely adopted by the community. In this review, the molecular methods and toolkits developed for S. cerevisiae are discussed in terms of their contributions to the required standardization efforts. In addition, the toolkits designed for emerging nonconventional yeast species including Yarrowia lipolytica, Komagataella phaffii, and Kluyveromyces marxianus are also reviewed. Without a doubt, the characterized DNA parts combined with the standardized assembly strategies highlighted in these toolkits have greatly contributed to the rapid development of many metabolic engineering and diagnostics applications among others. Despite the growing capacity in deploying synthetic biology for common yeast genome engineering works, the yeast community has a long journey to go to exploit it in more sophisticated and delicate applications like bioautomation | ||
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| 700 | 1 | |a Nowrouzi, Behnaz |e verfasserin |4 aut | |
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| 700 | 1 | |a Nascimento, Cibele Zolnier Sousa do |e verfasserin |4 aut | |
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| 700 | 1 | |a Elfick, Alistair |e verfasserin |4 aut | |
| 700 | 1 | |a Rios-Solis, Leonardo |e verfasserin |4 aut | |
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