Effect of Duration of Root Canal Infection on the Ability of Dentin-Pulp Complex Regeneration of Immature Permanent Teeth : An Animal Study
Copyright © 2022 American Association of Endodontists. Published by Elsevier Inc. All rights reserved..
INTRODUCTION: Persistent infection is always considered the most important reason for the failure of dentin-pulp complex regeneration. The present study aimed to evaluate the effect of the duration of root canal infection (from 1-12 weeks) on the ability of dentin-pulp complex regeneration.
METHODS: In this animal study, 64 roots of immature premolar teeth of 4 dogs were randomly divided into the following groups: the positive control group, 8 root canals treated with the regenerative endodontic procedure (REP); the negative control group, 12 infected root canals; the intervention groups, 36 root canals infected with supragingival plaque (1, 3, 6, and 12 weeks) and treated with REP; and an additional positive control group, 8 normal roots. After 3 months, the teeth were investigated by radiographic images and immunohistochemical staining (CD31, CD34, and S100 markers). In addition, DSPP gene expression was assessed using a real-time polymerase chain reaction technique.
RESULTS: Based on radiologic evaluation among the intervention groups, the highest root canal development (length and width) occurred in the intervention group of 1 week, and the lowest radiologic results were in the intervention groups of 6 and 12 weeks (1-way analysis of variance, P-value < .05). There was a significant difference between the groups in terms of CD31, CD34, S100, and DSPP expression percentage (1-way analysis of variance, P-value < .05); the highest and lowest expression percentages belonged to the 1- and 12-week groups, respectively, among the intervention groups.
CONCLUSIONS: This study demonstrated that long root canal infection decreased the ability of the body to regenerate the dentin-pulp complex.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:48 |
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| Enthalten in: |
Journal of endodontics - 48(2022), 10 vom: 03. Okt., Seite 1301-1307.e2 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Iranmanesh, Pedram [VerfasserIn] |
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| Links: |
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| Themen: |
Dental pulp |
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| Anmerkungen: |
Date Completed 28.09.2022 Date Revised 28.09.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.joen.2022.07.007 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2022 American Association of Endodontists. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a INTRODUCTION: Persistent infection is always considered the most important reason for the failure of dentin-pulp complex regeneration. The present study aimed to evaluate the effect of the duration of root canal infection (from 1-12 weeks) on the ability of dentin-pulp complex regeneration | ||
| 520 | |a METHODS: In this animal study, 64 roots of immature premolar teeth of 4 dogs were randomly divided into the following groups: the positive control group, 8 root canals treated with the regenerative endodontic procedure (REP); the negative control group, 12 infected root canals; the intervention groups, 36 root canals infected with supragingival plaque (1, 3, 6, and 12 weeks) and treated with REP; and an additional positive control group, 8 normal roots. After 3 months, the teeth were investigated by radiographic images and immunohistochemical staining (CD31, CD34, and S100 markers). In addition, DSPP gene expression was assessed using a real-time polymerase chain reaction technique | ||
| 520 | |a RESULTS: Based on radiologic evaluation among the intervention groups, the highest root canal development (length and width) occurred in the intervention group of 1 week, and the lowest radiologic results were in the intervention groups of 6 and 12 weeks (1-way analysis of variance, P-value < .05). There was a significant difference between the groups in terms of CD31, CD34, S100, and DSPP expression percentage (1-way analysis of variance, P-value < .05); the highest and lowest expression percentages belonged to the 1- and 12-week groups, respectively, among the intervention groups | ||
| 520 | |a CONCLUSIONS: This study demonstrated that long root canal infection decreased the ability of the body to regenerate the dentin-pulp complex | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Dental pulp | |
| 650 | 4 | |a infection | |
| 650 | 4 | |a regeneration | |
| 650 | 4 | |a regenerative endodontics | |
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| 700 | 1 | |a Saatchi, Masoud |e verfasserin |4 aut | |
| 700 | 1 | |a Toghraie, Davood |e verfasserin |4 aut | |
| 700 | 1 | |a Razavi, Sayed Mohammad |e verfasserin |4 aut | |
| 700 | 1 | |a Khademi, Abbasali |e verfasserin |4 aut | |
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