Biological activities and physicochemical characterization of alkaline lignins obtained from branches and leaves of Buchenavia viridiflora with potential pharmaceutical and biomedical applications
Copyright © 2022. Published by Elsevier B.V..
In this work, we investigated in vitro different biological activities of alkaline lignins extracted from the species Buchenavia viridiflora, a tree from the Amazon rainforest used as a wood product. The chemical composition results for the twig and leaves were, respectively (%): cellulose (30.88 and 24. 28), hemicellulose (21.62 and 23.03), lignin (29.93 and 25.46), extractives (13.06 and 20.52), and ash (4.51 and 6.72). The yield was higher for the lignin of the branches (67.9 %) when compared to the leaves (60.2 %). Lignins are of the GSH type, low molecular weight and thermally stable. They promoted moderate to low antioxidant activity, highlighting the lignin of the branches, which presented an IC50 of 884.56 μg/mL for the DPPH assay and an IC50 of 14.08 μg/mL for ABTS. In the cytotoxicity assays, they showed low toxicity against macrophage cells (IC50 28.47 and 22.58 μg/mL). In addition, they were not cytotoxic against splenocytes and erythrocytes at concentrations ranging from 100 to 6.25 μg/mL. These were able to promote splenocyte proliferation and induce the production of anti-inflammatory cytokines. And inhibit the growth of tumor cells with IC50 ranging from 12.63 to values >100 μg/mL and microbial at a concentration of 512 μg/mL. Finally, they showed antiparasitic activity by inhibiting the growth of chloroquine-sensitive and resistant Plasmodium falciparum strains. These findings reinforce that the lignins in this study are promising for potential pharmaceutical and biomedical applications.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:219 |
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| Enthalten in: |
International journal of biological macromolecules - 219(2022) vom: 31. Okt., Seite 224-245 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Araújo, Denise Maria Figueiredo [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 29.09.2022 Date Revised 29.09.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ijbiomac.2022.07.225 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM344462161 |
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| 100 | 1 | |a Araújo, Denise Maria Figueiredo |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Biological activities and physicochemical characterization of alkaline lignins obtained from branches and leaves of Buchenavia viridiflora with potential pharmaceutical and biomedical applications |
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| 500 | |a published: Print-Electronic | ||
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| 520 | |a Copyright © 2022. Published by Elsevier B.V. | ||
| 520 | |a In this work, we investigated in vitro different biological activities of alkaline lignins extracted from the species Buchenavia viridiflora, a tree from the Amazon rainforest used as a wood product. The chemical composition results for the twig and leaves were, respectively (%): cellulose (30.88 and 24. 28), hemicellulose (21.62 and 23.03), lignin (29.93 and 25.46), extractives (13.06 and 20.52), and ash (4.51 and 6.72). The yield was higher for the lignin of the branches (67.9 %) when compared to the leaves (60.2 %). Lignins are of the GSH type, low molecular weight and thermally stable. They promoted moderate to low antioxidant activity, highlighting the lignin of the branches, which presented an IC50 of 884.56 μg/mL for the DPPH assay and an IC50 of 14.08 μg/mL for ABTS. In the cytotoxicity assays, they showed low toxicity against macrophage cells (IC50 28.47 and 22.58 μg/mL). In addition, they were not cytotoxic against splenocytes and erythrocytes at concentrations ranging from 100 to 6.25 μg/mL. These were able to promote splenocyte proliferation and induce the production of anti-inflammatory cytokines. And inhibit the growth of tumor cells with IC50 ranging from 12.63 to values >100 μg/mL and microbial at a concentration of 512 μg/mL. Finally, they showed antiparasitic activity by inhibiting the growth of chloroquine-sensitive and resistant Plasmodium falciparum strains. These findings reinforce that the lignins in this study are promising for potential pharmaceutical and biomedical applications | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Amazon rainforest | |
| 650 | 4 | |a Biological activities | |
| 650 | 4 | |a Lignins | |
| 650 | 4 | |a Macromolecules | |
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| 650 | 7 | |a Antiparasitic Agents |2 NLM | |
| 650 | 7 | |a Cytokines |2 NLM | |
| 650 | 7 | |a Pharmaceutical Preparations |2 NLM | |
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| 700 | 1 | |a da Cruz Filho, Iranildo José |e verfasserin |4 aut | |
| 700 | 1 | |a Santos, Tiago |e verfasserin |4 aut | |
| 700 | 1 | |a Pereira, Daniel Tarciso Martins |e verfasserin |4 aut | |
| 700 | 1 | |a Marques, Diego Santa Clara |e verfasserin |4 aut | |
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| 700 | 1 | |a de Aquino, Thiago Mendonça |e verfasserin |4 aut | |
| 700 | 1 | |a de Moraes Rocha, George Jackson |e verfasserin |4 aut | |
| 700 | 1 | |a do Carmo Alves de Lima, Maria |e verfasserin |4 aut | |
| 700 | 1 | |a Nogueira, Fátima |e verfasserin |4 aut | |
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