Fluoride and trimetaphosphate association as a novel approach for remineralization and antiproteolytic activity in dentin tissue
Copyright © 2022 Elsevier Ltd. All rights reserved..
OBJECTIVE: The study evaluated the effect of solutions containing fluoride (F) and/or sodium trimetaphosphate (TMP) and F/TMP on the inhibition of MMP-2 and MMP-9, and on dentin remineralization in vitro.
DESIGN: Bovine root dentin blocks were prepared, and caries-like lesions were induced in two thirds of the surface. Blocks were then randomly divided into 13 groups/solutions (n = 10): Placebo; 0.3 %, 1 % and 3 % NaOH-hydrolyzed TMP; 0.3 %, 1 % and 3 % TMP; 250, 500 and 1100 ppm F; 250 ppm F + 0.3 % TMP; 500 ppm F + 1 % TMP and 1100 ppm F + 3 % TMP. One third of each specimen was treated with the respective solutions in pH-cycling. The mineral concentration (gHAp × cm-3 × µm) was determined by computed X-ray microtomography, and data submitted to ANOVA and Student-Newman-Keuls' test (p < 0.05). The ability of the solutions to inhibit MMP-2 and MMP-9 activity was assessed by zymography.
RESULTS: F/TMP association led to less mineral loss in the deeper region of the lesion and reduced the depth of lesions when compared to its counterpart without TMP (p < 0.001). 3 % TMP (hydrolyzed or not), 500 ppm F and 1100 ppm F completely inhibited MMP-2 activity, while for MMP-9 such effects were only achieved by treatment with 1100 ppm F + 3 % TMP.
CONCLUSION: Treatment with 1100 ppm F + 3 % TMP fully inhibits the gelatinolytic activity of MMPs-2 and - 9 and shows greater remineralizing capacity in artificial caries lesions in dentin. However, hydrolyzing TMP does not improve its anti-proteolytic activity and its remineralizing capacity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:142 |
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| Enthalten in: |
Archives of oral biology - 142(2022) vom: 01. Okt., Seite 105508 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Nunes, Gabriel Pereira [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 08.09.2022 Date Revised 12.09.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.archoralbio.2022.105508 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 100 | 1 | |a Nunes, Gabriel Pereira |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Fluoride and trimetaphosphate association as a novel approach for remineralization and antiproteolytic activity in dentin tissue |
| 264 | 1 | |c 2022 | |
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| 500 | |a Date Revised 12.09.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2022 Elsevier Ltd. All rights reserved. | ||
| 520 | |a OBJECTIVE: The study evaluated the effect of solutions containing fluoride (F) and/or sodium trimetaphosphate (TMP) and F/TMP on the inhibition of MMP-2 and MMP-9, and on dentin remineralization in vitro | ||
| 520 | |a DESIGN: Bovine root dentin blocks were prepared, and caries-like lesions were induced in two thirds of the surface. Blocks were then randomly divided into 13 groups/solutions (n = 10): Placebo; 0.3 %, 1 % and 3 % NaOH-hydrolyzed TMP; 0.3 %, 1 % and 3 % TMP; 250, 500 and 1100 ppm F; 250 ppm F + 0.3 % TMP; 500 ppm F + 1 % TMP and 1100 ppm F + 3 % TMP. One third of each specimen was treated with the respective solutions in pH-cycling. The mineral concentration (gHAp × cm-3 × µm) was determined by computed X-ray microtomography, and data submitted to ANOVA and Student-Newman-Keuls' test (p < 0.05). The ability of the solutions to inhibit MMP-2 and MMP-9 activity was assessed by zymography | ||
| 520 | |a RESULTS: F/TMP association led to less mineral loss in the deeper region of the lesion and reduced the depth of lesions when compared to its counterpart without TMP (p < 0.001). 3 % TMP (hydrolyzed or not), 500 ppm F and 1100 ppm F completely inhibited MMP-2 activity, while for MMP-9 such effects were only achieved by treatment with 1100 ppm F + 3 % TMP | ||
| 520 | |a CONCLUSION: Treatment with 1100 ppm F + 3 % TMP fully inhibits the gelatinolytic activity of MMPs-2 and - 9 and shows greater remineralizing capacity in artificial caries lesions in dentin. However, hydrolyzing TMP does not improve its anti-proteolytic activity and its remineralizing capacity | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Randomized Controlled Trial | |
| 650 | 4 | |a Dentin | |
| 650 | 4 | |a Fluoride | |
| 650 | 4 | |a Matrix metalloproteinases | |
| 650 | 4 | |a Phosphate | |
| 650 | 4 | |a Remineralization | |
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| 650 | 7 | |a Minerals |2 NLM | |
| 650 | 7 | |a Sodium Fluoride |2 NLM | |
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| 700 | 1 | |a Danelon, Marcelle |e verfasserin |4 aut | |
| 700 | 1 | |a Pessan, Juliano Pelim |e verfasserin |4 aut | |
| 700 | 1 | |a Capalbo, Letícia Cabrera |e verfasserin |4 aut | |
| 700 | 1 | |a Junior, Nilson Antônio Nunes |e verfasserin |4 aut | |
| 700 | 1 | |a Matos, Adriana Arruda |e verfasserin |4 aut | |
| 700 | 1 | |a Souza, José Antonio Santos |e verfasserin |4 aut | |
| 700 | 1 | |a Buzalaf, Marília Afonso Rabelo |e verfasserin |4 aut | |
| 700 | 1 | |a Delbem, Alberto Carlos Botazzo |e verfasserin |4 aut | |
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