Regulatory roles of NAT10 in airway epithelial cell function and metabolism in pathological conditions
© 2022. The Author(s), under exclusive licence to Springer Nature B.V..
N-acetyltransferase 10 (NAT10), a nuclear acetyltransferase and a member of the GNAT family, plays critical roles in RNA stability and translation processes as well as cell proliferation. Little is known about regulatory effects of NAT10 in lung epithelial cell proliferation. We firstly investigated NTA10 mRNA expression in alveolar epithelial types I and II, basal, ciliated, club, and goblet/mucous epithelia from heathy and patients with chronic obstructive pulmonary disease, idiopathic pulmonary fibrosis, lung adenocarcinoma, para-tumor tissue, and systemic sclerosis, respectively. We selected A549 cells for representative of alveolar epithelia or H1299 and H460 cells as airway epithelia with different genetic backgrounds and studied dynamic responses of NAT10-down-regulated epithelia to high temperature, lipopolysaccharide, cigarette smoking extract (CSE), drugs, radiation, and phosphoinositide 3-kinase (PI3K) inhibitors at various doses. We also compared transcriptomic profiles between alveolar and airway epithelia, between cells with or without NAT10 down-regulation, between early and late stages, and between challenges. The present study demonstrated that NAT10 expression increased in human lung epithelia and varied among epithelial types, challenges, and diseases. Knockdown of NAT10 altered epithelial mitochondrial functions, dynamic responses to LPS, CSE, or PI3K inhibitors, and transcriptomic phenomes. NAT10 regulates biological phenomes, and behaviors are more complex and are dependent upon multiple signal pathways. Thus, NAT10-associated signal pathways can be a new alternative for understanding the disease and developing new biomarkers and targets.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:39 |
|---|---|
| Enthalten in: |
Cell biology and toxicology - 39(2023), 4 vom: 25. Aug., Seite 1237-1256 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Zheng, Nannan [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 16.08.2023 Date Revised 20.08.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1007/s10565-022-09743-z |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM343969211 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM343969211 | ||
| 003 | DE-627 | ||
| 005 | 20231226021657.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s10565-022-09743-z |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1146.xml |
| 035 | |a (DE-627)NLM343969211 | ||
| 035 | |a (NLM)35877022 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Zheng, Nannan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Regulatory roles of NAT10 in airway epithelial cell function and metabolism in pathological conditions |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 16.08.2023 | ||
| 500 | |a Date Revised 20.08.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2022. The Author(s), under exclusive licence to Springer Nature B.V. | ||
| 520 | |a N-acetyltransferase 10 (NAT10), a nuclear acetyltransferase and a member of the GNAT family, plays critical roles in RNA stability and translation processes as well as cell proliferation. Little is known about regulatory effects of NAT10 in lung epithelial cell proliferation. We firstly investigated NTA10 mRNA expression in alveolar epithelial types I and II, basal, ciliated, club, and goblet/mucous epithelia from heathy and patients with chronic obstructive pulmonary disease, idiopathic pulmonary fibrosis, lung adenocarcinoma, para-tumor tissue, and systemic sclerosis, respectively. We selected A549 cells for representative of alveolar epithelia or H1299 and H460 cells as airway epithelia with different genetic backgrounds and studied dynamic responses of NAT10-down-regulated epithelia to high temperature, lipopolysaccharide, cigarette smoking extract (CSE), drugs, radiation, and phosphoinositide 3-kinase (PI3K) inhibitors at various doses. We also compared transcriptomic profiles between alveolar and airway epithelia, between cells with or without NAT10 down-regulation, between early and late stages, and between challenges. The present study demonstrated that NAT10 expression increased in human lung epithelia and varied among epithelial types, challenges, and diseases. Knockdown of NAT10 altered epithelial mitochondrial functions, dynamic responses to LPS, CSE, or PI3K inhibitors, and transcriptomic phenomes. NAT10 regulates biological phenomes, and behaviors are more complex and are dependent upon multiple signal pathways. Thus, NAT10-associated signal pathways can be a new alternative for understanding the disease and developing new biomarkers and targets | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Epithelial cells | |
| 650 | 4 | |a LPS | |
| 650 | 4 | |a Lung diseases | |
| 650 | 4 | |a N-acetyltransferase 10 | |
| 650 | 4 | |a Smoking | |
| 650 | 4 | |a Transcriptomics | |
| 650 | 7 | |a Phosphatidylinositol 3-Kinases |2 NLM | |
| 650 | 7 | |a EC 2.7.1.- |2 NLM | |
| 650 | 7 | |a Acetyltransferases |2 NLM | |
| 650 | 7 | |a EC 2.3.1.- |2 NLM | |
| 650 | 7 | |a NAT10 protein, human |2 NLM | |
| 650 | 7 | |a EC 2.3.1.88 |2 NLM | |
| 650 | 7 | |a N-Terminal Acetyltransferases |2 NLM | |
| 650 | 7 | |a EC 2.3.1.88 |2 NLM | |
| 700 | 1 | |a Liu, Xuanqi |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yifei |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Furong |e verfasserin |4 aut | |
| 700 | 1 | |a Zeng, Yiming |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Yunfeng |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Duojiao |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Chengshui |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiangdong |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cell biology and toxicology |d 1984 |g 39(2023), 4 vom: 25. Aug., Seite 1237-1256 |w (DE-627)NLM012983675 |x 1573-6822 |7 nnns |
| 773 | 1 | 8 | |g volume:39 |g year:2023 |g number:4 |g day:25 |g month:08 |g pages:1237-1256 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s10565-022-09743-z |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 39 |j 2023 |e 4 |b 25 |c 08 |h 1237-1256 | ||