Combinatorial Gli activity directs immune infiltration and tumor growth in pancreatic cancer
Proper Hedgehog (HH) signaling is essential for embryonic development, while aberrant HH signaling drives pediatric and adult cancers. HH signaling is frequently dysregulated in pancreatic cancer, yet its role remains controversial, with both tumor-promoting and tumor-restraining functions reported. Notably, the GLI family of HH transcription factors (GLI1, GLI2, GLI3), remain largely unexplored in pancreatic cancer. We therefore investigated the individual and combined contributions of GLI1-3 to pancreatic cancer progression. At pre-cancerous stages, fibroblast-specific Gli2/Gli3 deletion decreases immunosuppressive macrophage infiltration and promotes T cell infiltration. Strikingly, combined loss of Gli1/Gli2/Gli3 promotes macrophage infiltration, indicating that subtle changes in Gli expression differentially regulate immune infiltration. In invasive tumors, Gli2/Gli3 KO fibroblasts exclude immunosuppressive myeloid cells and suppress tumor growth by recruiting natural killer cells. Finally, we demonstrate that fibroblasts directly regulate macrophage and T cell migration through the expression of Gli-dependent cytokines. Thus, the coordinated activity of GLI1-3 directs the fibroinflammatory response throughout pancreatic cancer progression.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:18 |
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| Enthalten in: |
PLoS genetics - 18(2022), 7 vom: 12. Juli, Seite e1010315 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Scales, Michael K [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 05.08.2022 Date Revised 21.10.2023 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.1371/journal.pgen.1010315 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM343877228 |
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| 100 | 1 | |a Scales, Michael K |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Combinatorial Gli activity directs immune infiltration and tumor growth in pancreatic cancer |
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| 500 | |a Date Revised 21.10.2023 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Proper Hedgehog (HH) signaling is essential for embryonic development, while aberrant HH signaling drives pediatric and adult cancers. HH signaling is frequently dysregulated in pancreatic cancer, yet its role remains controversial, with both tumor-promoting and tumor-restraining functions reported. Notably, the GLI family of HH transcription factors (GLI1, GLI2, GLI3), remain largely unexplored in pancreatic cancer. We therefore investigated the individual and combined contributions of GLI1-3 to pancreatic cancer progression. At pre-cancerous stages, fibroblast-specific Gli2/Gli3 deletion decreases immunosuppressive macrophage infiltration and promotes T cell infiltration. Strikingly, combined loss of Gli1/Gli2/Gli3 promotes macrophage infiltration, indicating that subtle changes in Gli expression differentially regulate immune infiltration. In invasive tumors, Gli2/Gli3 KO fibroblasts exclude immunosuppressive myeloid cells and suppress tumor growth by recruiting natural killer cells. Finally, we demonstrate that fibroblasts directly regulate macrophage and T cell migration through the expression of Gli-dependent cytokines. Thus, the coordinated activity of GLI1-3 directs the fibroinflammatory response throughout pancreatic cancer progression | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Hedgehog Proteins |2 NLM | |
| 650 | 7 | |a Kruppel-Like Transcription Factors |2 NLM | |
| 650 | 7 | |a Nerve Tissue Proteins |2 NLM | |
| 650 | 7 | |a Zinc Finger Protein GLI1 |2 NLM | |
| 650 | 7 | |a Zinc Finger Protein Gli2 |2 NLM | |
| 650 | 7 | |a Zinc Finger Protein Gli3 |2 NLM | |
| 700 | 1 | |a Velez-Delgado, Ashley |e verfasserin |4 aut | |
| 700 | 1 | |a Steele, Nina G |e verfasserin |4 aut | |
| 700 | 1 | |a Schrader, Hannah E |e verfasserin |4 aut | |
| 700 | 1 | |a Stabnick, Anna M |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Mercado Soto, Nayanna M |e verfasserin |4 aut | |
| 700 | 1 | |a Nwosu, Zeribe C |e verfasserin |4 aut | |
| 700 | 1 | |a Johnson, Craig |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yaqing |e verfasserin |4 aut | |
| 700 | 1 | |a Salas-Escabillas, Daniel J |e verfasserin |4 aut | |
| 700 | 1 | |a Menjivar, Rosa E |e verfasserin |4 aut | |
| 700 | 1 | |a Maurer, H Carlo |e verfasserin |4 aut | |
| 700 | 1 | |a Crawford, Howard C |e verfasserin |4 aut | |
| 700 | 1 | |a Bednar, Filip |e verfasserin |4 aut | |
| 700 | 1 | |a Olive, Kenneth P |e verfasserin |4 aut | |
| 700 | 1 | |a Pasca di Magliano, Marina |e verfasserin |4 aut | |
| 700 | 1 | |a Allen, Benjamin L |e verfasserin |4 aut | |
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