Persistence of MERS-CoV-spike-specific B cells and antibodies after late third immunization with the MVA-MERS-S vaccine
Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved..
The Middle East respiratory syndrome (MERS) is a respiratory disease caused by MERS coronavirus (MERS-CoV). In follow up to a phase 1 trial, we perform a longitudinal analysis of immune responses following immunization with the modified vaccinia virus Ankara (MVA)-based vaccine MVA-MERS-S encoding the MERS-CoV-spike protein. Three homologous immunizations were administered on days 0 and 28 with a late booster vaccination at 12 ± 4 months. Antibody isotypes, subclasses, and neutralization capacity as well as T and B cell responses were monitored over a period of 3 years using standard and bead-based enzyme-linked immunosorbent assay (ELISA), 50% plaque-reduction neutralization test (PRNT50), enzyme-linked immunospot (ELISpot), and flow cytometry. The late booster immunization significantly increases the frequency and persistence of spike-specific B cells, binding immunoglobulin G1 (IgG1) and neutralizing antibodies but not T cell responses. Our data highlight the potential of a late boost to enhance long-term antibody and B cell immunity against MERS-CoV. Our findings on the MVA-MERS-S vaccine may be of relevance for coronavirus 2019 (COVID-19) vaccination strategies.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2022 |
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Erschienen: |
2022 |
Enthalten in: |
Zur Gesamtaufnahme - volume:3 |
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Enthalten in: |
Cell reports. Medicine - 3(2022), 7 vom: 19. Juli, Seite 100685 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Weskamm, Leonie M [VerfasserIn] |
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Links: |
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Anmerkungen: |
Date Completed 22.07.2022 Date Revised 08.09.2022 published: Print ClinicalTrials.gov: NCT03615911 Citation Status MEDLINE |
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doi: |
10.1016/j.xcrm.2022.100685 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM343786427 |
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245 | 1 | 0 | |a Persistence of MERS-CoV-spike-specific B cells and antibodies after late third immunization with the MVA-MERS-S vaccine |
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500 | |a published: Print | ||
500 | |a ClinicalTrials.gov: NCT03615911 | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved. | ||
520 | |a The Middle East respiratory syndrome (MERS) is a respiratory disease caused by MERS coronavirus (MERS-CoV). In follow up to a phase 1 trial, we perform a longitudinal analysis of immune responses following immunization with the modified vaccinia virus Ankara (MVA)-based vaccine MVA-MERS-S encoding the MERS-CoV-spike protein. Three homologous immunizations were administered on days 0 and 28 with a late booster vaccination at 12 ± 4 months. Antibody isotypes, subclasses, and neutralization capacity as well as T and B cell responses were monitored over a period of 3 years using standard and bead-based enzyme-linked immunosorbent assay (ELISA), 50% plaque-reduction neutralization test (PRNT50), enzyme-linked immunospot (ELISpot), and flow cytometry. The late booster immunization significantly increases the frequency and persistence of spike-specific B cells, binding immunoglobulin G1 (IgG1) and neutralizing antibodies but not T cell responses. Our data highlight the potential of a late boost to enhance long-term antibody and B cell immunity against MERS-CoV. Our findings on the MVA-MERS-S vaccine may be of relevance for coronavirus 2019 (COVID-19) vaccination strategies | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a IgG ELISpot | |
650 | 4 | |a IgG subclasses | |
650 | 4 | |a MERS-CoV | |
650 | 4 | |a MVA-MERS-S | |
650 | 4 | |a immune persistence | |
650 | 4 | |a late booster vaccination | |
650 | 4 | |a long-term immunity | |
650 | 4 | |a memory B cells | |
650 | 4 | |a neutralizing antibodies | |
650 | 4 | |a viral vector vaccine | |
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700 | 1 | |a Fathi, Anahita |e verfasserin |4 aut | |
700 | 1 | |a Raadsen, Matthijs P |e verfasserin |4 aut | |
700 | 1 | |a Mykytyn, Anna Z |e verfasserin |4 aut | |
700 | 1 | |a Koch, Till |e verfasserin |4 aut | |
700 | 1 | |a Spohn, Michael |e verfasserin |4 aut | |
700 | 1 | |a Friedrich, Monika |e verfasserin |4 aut | |
700 | 0 | |a MVA-MERS-S Study Group |e verfasserin |4 aut | |
700 | 1 | |a Haagmans, Bart L |e verfasserin |4 aut | |
700 | 1 | |a Becker, Stephan |e verfasserin |4 aut | |
700 | 1 | |a Sutter, Gerd |e verfasserin |4 aut | |
700 | 1 | |a Dahlke, Christine |e verfasserin |4 aut | |
700 | 1 | |a Addo, Marylyn M |e verfasserin |4 aut | |
700 | 1 | |a Bartels, Etienne |e investigator |4 oth | |
700 | 1 | |a Gundlach, Swantje |e investigator |4 oth | |
700 | 1 | |a Hesterkamp, Thomas |e investigator |4 oth | |
700 | 1 | |a Krähling, Verena |e investigator |4 oth | |
700 | 1 | |a Lassen, Susan |e investigator |4 oth | |
700 | 1 | |a Ly, My Linh |e investigator |4 oth | |
700 | 1 | |a Pötsch, Joseph H |e investigator |4 oth | |
700 | 1 | |a Schmiedel, Stefan |e investigator |4 oth | |
700 | 1 | |a Volz, Asisa |e investigator |4 oth | |
700 | 1 | |a Zinser, Madeleine E |e investigator |4 oth | |
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