Comparative study of dedifferentiated fat cell and adipose-derived stromal cell sheets for periodontal tissue regeneration : In vivo and in vitro evidence
© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd..
AIM: To compare the efficacy of adipocyte-derived dedifferentiated fat (DFAT) cell and adipose-derived stromal cell (ADSC) sheets for regenerative treatment of intra-bony periodontal defects.
MATERIALS AND METHODS: DFAT cells were obtained using the ceiling culture method and were compared with ADSCs using Cell Counting Kit-8, colony formation assay, surface antigen identification, and multilineage differentiation assays. DFAT and ADSC sheets were prepared in a cell sheet culture medium. The biological characteristics of DFAT cell and ADSC sheets were compared using haematoxylin and eosin staining, quantitative reverse transcription polymerase chain reaction, and immunofluorescence staining. Micro-computed tomography and histological staining were used to compare the effects of the two cell sheets on the repair of periodontal intra-bony defects in rats.
RESULTS: DFAT cells and ADSCs demonstrated mesenchymal stem cell characteristics. Both cell types were CD29-, CD90-, and CD146-positive and CD31-, CD34-, and CD45-negative. DFAT cells and ADSCs exhibited similar osteogenic and adipogenic differentiation capabilities and colony formation ability. DFAT cells displayed stronger proliferation capabilities compared with ADSCs. Compared with the ADSC sheets, DFAT cell sheets exhibited a higher expression of periodontal-related genes and proteins and greater ability to regenerate periodontal tissue.
CONCLUSIONS: Our findings suggest that DFAT cell sheets are an ideal seed cell source and form of cell delivery for periodontal intra-bony defects.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:49 |
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| Enthalten in: |
Journal of clinical periodontology - 49(2022), 12 vom: 18. Dez., Seite 1289-1303 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Huang, Guobin [VerfasserIn] |
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| Links: |
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| Themen: |
Adipose-derived stromal cells |
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| Anmerkungen: |
Date Completed 11.11.2022 Date Revised 17.11.2022 published: Print-Electronic figshare: 10.6084/m9.figshare.20394153 Citation Status MEDLINE |
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| doi: |
10.1111/jcpe.13705 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 100 | 1 | |a Huang, Guobin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Comparative study of dedifferentiated fat cell and adipose-derived stromal cell sheets for periodontal tissue regeneration |b In vivo and in vitro evidence |
| 264 | 1 | |c 2022 | |
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| 500 | |a Date Completed 11.11.2022 | ||
| 500 | |a Date Revised 17.11.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a figshare: 10.6084/m9.figshare.20394153 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. | ||
| 520 | |a AIM: To compare the efficacy of adipocyte-derived dedifferentiated fat (DFAT) cell and adipose-derived stromal cell (ADSC) sheets for regenerative treatment of intra-bony periodontal defects | ||
| 520 | |a MATERIALS AND METHODS: DFAT cells were obtained using the ceiling culture method and were compared with ADSCs using Cell Counting Kit-8, colony formation assay, surface antigen identification, and multilineage differentiation assays. DFAT and ADSC sheets were prepared in a cell sheet culture medium. The biological characteristics of DFAT cell and ADSC sheets were compared using haematoxylin and eosin staining, quantitative reverse transcription polymerase chain reaction, and immunofluorescence staining. Micro-computed tomography and histological staining were used to compare the effects of the two cell sheets on the repair of periodontal intra-bony defects in rats | ||
| 520 | |a RESULTS: DFAT cells and ADSCs demonstrated mesenchymal stem cell characteristics. Both cell types were CD29-, CD90-, and CD146-positive and CD31-, CD34-, and CD45-negative. DFAT cells and ADSCs exhibited similar osteogenic and adipogenic differentiation capabilities and colony formation ability. DFAT cells displayed stronger proliferation capabilities compared with ADSCs. Compared with the ADSC sheets, DFAT cell sheets exhibited a higher expression of periodontal-related genes and proteins and greater ability to regenerate periodontal tissue | ||
| 520 | |a CONCLUSIONS: Our findings suggest that DFAT cell sheets are an ideal seed cell source and form of cell delivery for periodontal intra-bony defects | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a adipose-derived stromal cells | |
| 650 | 4 | |a cell sheet | |
| 650 | 4 | |a dedifferentiated fat cells | |
| 650 | 4 | |a periodontitis | |
| 650 | 4 | |a tissue regeneration | |
| 700 | 1 | |a Xia, Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Dai, Zichao |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Rongqiang |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Rui |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Hefeng |e verfasserin |4 aut | |
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