Hypoxia, a dynamic tool to amplify the gingival mesenchymal stem cells potential for neurotrophic factor secretion
© 2022 The Author(s)..
Gingival mesenchymal stem cells (GMSCs) have significant regenerative potential. Their potential applications range from the treatment of inflammatory diseases, wound healing, and oral disorders. Preconditioning these stem cells can optimize their biological properties. Hypoxia preconditioning of MSCs improves stem cell properties like proliferation, survival, and differentiation potential. This research explored the possible impact of hypoxia on the pluripotent stem cell properties that GMSCs possess. We evaluated the morphology, stemness, neurotrophic factors, and stemness-related genes. We compared the protein levels of secreted neurotrophic factors between normoxic and hypoxic GMSC-conditioned media (GMSC-CM). Results revealed that hypoxic cultured GMSC's had augmented expression of neurotrophic factors BDNF, GDNF, VEGF, and IGF1 and stemness-related gene NANOG. Hypoxic GMSCs showed decreased expression of the OCT4 gene. In hypoxic GMSC-CM, the neurotrophic factors secretions were significantly higher than normoxic GMSC-CM. Our data demonstrate that culturing of GMSCs in hypoxia enhances the secretion of neurotrophic factors that can lead to neuronal lineage differentiation.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:29 |
|---|---|
| Enthalten in: |
Saudi journal of biological sciences - 29(2022), 5 vom: 17. Mai, Seite 3568-3576 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Patil, Shankargouda [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Gingival mesenchymal stem cell |
|---|
| Anmerkungen: |
Date Revised 19.07.2022 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.1016/j.sjbs.2022.02.039 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM343646382 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM343646382 | ||
| 003 | DE-627 | ||
| 005 | 20231226020933.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.sjbs.2022.02.039 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1145.xml |
| 035 | |a (DE-627)NLM343646382 | ||
| 035 | |a (NLM)35844419 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Patil, Shankargouda |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Hypoxia, a dynamic tool to amplify the gingival mesenchymal stem cells potential for neurotrophic factor secretion |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 19.07.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2022 The Author(s). | ||
| 520 | |a Gingival mesenchymal stem cells (GMSCs) have significant regenerative potential. Their potential applications range from the treatment of inflammatory diseases, wound healing, and oral disorders. Preconditioning these stem cells can optimize their biological properties. Hypoxia preconditioning of MSCs improves stem cell properties like proliferation, survival, and differentiation potential. This research explored the possible impact of hypoxia on the pluripotent stem cell properties that GMSCs possess. We evaluated the morphology, stemness, neurotrophic factors, and stemness-related genes. We compared the protein levels of secreted neurotrophic factors between normoxic and hypoxic GMSC-conditioned media (GMSC-CM). Results revealed that hypoxic cultured GMSC's had augmented expression of neurotrophic factors BDNF, GDNF, VEGF, and IGF1 and stemness-related gene NANOG. Hypoxic GMSCs showed decreased expression of the OCT4 gene. In hypoxic GMSC-CM, the neurotrophic factors secretions were significantly higher than normoxic GMSC-CM. Our data demonstrate that culturing of GMSCs in hypoxia enhances the secretion of neurotrophic factors that can lead to neuronal lineage differentiation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Gingival mesenchymal stem cell | |
| 650 | 4 | |a Hypoxia | |
| 650 | 4 | |a Neurotrophic factors | |
| 650 | 4 | |a Normoxia | |
| 700 | 1 | |a Fageeh, Hytham N |e verfasserin |4 aut | |
| 700 | 1 | |a Fageeh, Hammam Ibrahim |e verfasserin |4 aut | |
| 700 | 1 | |a Ibraheem, Wael |e verfasserin |4 aut | |
| 700 | 1 | |a Alshehri, Abdulrahman Saleh |e verfasserin |4 aut | |
| 700 | 1 | |a Al-Brakati, Ashraf |e verfasserin |4 aut | |
| 700 | 1 | |a Almoammar, Salem |e verfasserin |4 aut | |
| 700 | 1 | |a Almagbol, Mohammad |e verfasserin |4 aut | |
| 700 | 1 | |a Dewan, Harisha |e verfasserin |4 aut | |
| 700 | 1 | |a Khan, Samar Saeed |e verfasserin |4 aut | |
| 700 | 1 | |a Baeshen, Hosam Ali |e verfasserin |4 aut | |
| 700 | 1 | |a Patil, Vikrant R |e verfasserin |4 aut | |
| 700 | 1 | |a Raj, A Thirumal |e verfasserin |4 aut | |
| 700 | 1 | |a Bhandi, Shilpa |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Saudi journal of biological sciences |d 2009 |g 29(2022), 5 vom: 17. Mai, Seite 3568-3576 |w (DE-627)NLM23017549X |x 1319-562X |7 nnns |
| 773 | 1 | 8 | |g volume:29 |g year:2022 |g number:5 |g day:17 |g month:05 |g pages:3568-3576 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.sjbs.2022.02.039 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 29 |j 2022 |e 5 |b 17 |c 05 |h 3568-3576 | ||