Chromosome-level genome assembly defines female-biased genes associated with sex determination and differentiation in the human blood fluke Schistosoma japonicum
© 2022 John Wiley & Sons Ltd..
Schistosomiasis is a neglected tropical disease of humans caused by blood flukes of the genus Schistosoma, the only dioecious parasitic flatworm. Although aspects of sex determination, differentiation and reproduction have been studied in some Schistosoma species, almost nothing is known for Schistosoma japonicum, the causative agent of schistosomiasis japonica. This mainly reflects the lack of high-quality genomic and transcriptomic resources for this species. As current genomes for S. japonicum are highly fragmented, we assembled and report a chromosome-level reference genome (seven autosomes, the Z-chromosome and partial W-chromosome), achieving a substantially enhanced gene annotation. Utilizing this genome, we discovered that the sex chromosomes of S. japonicum and its congener S. mansoni independently suppressed recombination during evolution, forming five and two evolutionary strata, respectively. By exploring the W-chromosome and sex-specific transcriptomes, we identified 35 W-linked genes and 257 female-preferentially transcribed genes (FTGs) from our chromosomal assembly and uncovered a signature for sex determination and differentiation in S. japonicum. These FTGs clustering within autosomes or the Z-chromosome exhibit a highly dynamic transcription profile during the pairing of female and male schistosomula, thereby representing a critical phase for the maturation of the female worms and suggesting distinct layers of regulatory control of gene transcription at this development stage. Collectively, these data provide a valuable resource for further functional genomic characterization of S. japonicum, shed light on the evolution of sex chromosomes in this highly virulent human blood fluke, and provide a pathway to identify novel targets for development of intervention tools against schistosomiasis.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:23 |
|---|---|
| Enthalten in: |
Molecular ecology resources - 23(2023), 1 vom: 01. Jan., Seite 205-221 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Xu, Xindong [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Genome assembly |
|---|
| Anmerkungen: |
Date Completed 06.12.2022 Date Revised 06.12.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1111/1755-0998.13689 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM34364276X |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM34364276X | ||
| 003 | DE-627 | ||
| 005 | 20231226020928.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/1755-0998.13689 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1145.xml |
| 035 | |a (DE-627)NLM34364276X | ||
| 035 | |a (NLM)35844053 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Xu, Xindong |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Chromosome-level genome assembly defines female-biased genes associated with sex determination and differentiation in the human blood fluke Schistosoma japonicum |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 06.12.2022 | ||
| 500 | |a Date Revised 06.12.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2022 John Wiley & Sons Ltd. | ||
| 520 | |a Schistosomiasis is a neglected tropical disease of humans caused by blood flukes of the genus Schistosoma, the only dioecious parasitic flatworm. Although aspects of sex determination, differentiation and reproduction have been studied in some Schistosoma species, almost nothing is known for Schistosoma japonicum, the causative agent of schistosomiasis japonica. This mainly reflects the lack of high-quality genomic and transcriptomic resources for this species. As current genomes for S. japonicum are highly fragmented, we assembled and report a chromosome-level reference genome (seven autosomes, the Z-chromosome and partial W-chromosome), achieving a substantially enhanced gene annotation. Utilizing this genome, we discovered that the sex chromosomes of S. japonicum and its congener S. mansoni independently suppressed recombination during evolution, forming five and two evolutionary strata, respectively. By exploring the W-chromosome and sex-specific transcriptomes, we identified 35 W-linked genes and 257 female-preferentially transcribed genes (FTGs) from our chromosomal assembly and uncovered a signature for sex determination and differentiation in S. japonicum. These FTGs clustering within autosomes or the Z-chromosome exhibit a highly dynamic transcription profile during the pairing of female and male schistosomula, thereby representing a critical phase for the maturation of the female worms and suggesting distinct layers of regulatory control of gene transcription at this development stage. Collectively, these data provide a valuable resource for further functional genomic characterization of S. japonicum, shed light on the evolution of sex chromosomes in this highly virulent human blood fluke, and provide a pathway to identify novel targets for development of intervention tools against schistosomiasis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Schistosoma japonicum | |
| 650 | 4 | |a genome assembly | |
| 650 | 4 | |a schistosomiasis | |
| 650 | 4 | |a sex chromosomes | |
| 650 | 4 | |a sex differentiation | |
| 700 | 1 | |a Wang, Yifeng |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Changhong |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Gangqiang |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Xinyu |e verfasserin |4 aut | |
| 700 | 1 | |a Dai, Yang |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yaobao |e verfasserin |4 aut | |
| 700 | 1 | |a Wei, Guiying |e verfasserin |4 aut | |
| 700 | 1 | |a He, Xiaohui |e verfasserin |4 aut | |
| 700 | 1 | |a Jin, Ge |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Ziqiu |e verfasserin |4 aut | |
| 700 | 1 | |a Guan, Qingtian |e verfasserin |4 aut | |
| 700 | 1 | |a Pain, Arnab |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Shengyue |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Wenbao |e verfasserin |4 aut | |
| 700 | 1 | |a Young, Neil D |e verfasserin |4 aut | |
| 700 | 1 | |a Gasser, Robin B |e verfasserin |4 aut | |
| 700 | 1 | |a McManus, Donald P |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Qi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Qingfeng |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular ecology resources |d 2008 |g 23(2023), 1 vom: 01. Jan., Seite 205-221 |w (DE-627)NLM206871481 |x 1755-0998 |7 nnns |
| 773 | 1 | 8 | |g volume:23 |g year:2023 |g number:1 |g day:01 |g month:01 |g pages:205-221 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/1755-0998.13689 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 23 |j 2023 |e 1 |b 01 |c 01 |h 205-221 | ||