3D-printed SAXS chamber for controlled in situ dialysis and optical characterization
open access..
3D printing changes the scope of how samples can be mounted for small-angle X-ray scattering (SAXS). In this paper a 3D-printed X-ray chamber, which allows for in situ exchange of buffer and in situ optical transmission spectroscopy, is presented. The chamber is made of cyclic olefin copolymers (COC), including COC X-ray windows providing ultra-low SAXS background. The design integrates a membrane insert for in situ dialysis of the 100 µl sample volume against a reservoir, which enables measurements of the same sample under multiple conditions using an in-house X-ray setup equipped with a 17.4 keV molybdenum source. The design's capabilities are demonstrated by measuring reversible structural changes in lipid and polymer systems as a function of salt concentration and pH. In the same chambers optical light transmission spectroscopy was carried out measuring the optical turbidity of the mesophases and local pH values using pH-responsive dyes. Microfluidic exchange and optical spectroscopy combined with in situ X-ray scattering enables vast applications for the study of responsive materials.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:29 |
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| Enthalten in: |
Journal of synchrotron radiation - 29(2022), Pt 4 vom: 01. Juli, Seite 1014-1019 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ehm, Tamara [VerfasserIn] |
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| Links: |
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| Themen: |
3D printing |
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| Anmerkungen: |
Date Completed 06.07.2022 Date Revised 16.07.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1107/S1600577522005136 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM343082489 |
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| 500 | |a Date Revised 16.07.2022 | ||
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a open access. | ||
| 520 | |a 3D printing changes the scope of how samples can be mounted for small-angle X-ray scattering (SAXS). In this paper a 3D-printed X-ray chamber, which allows for in situ exchange of buffer and in situ optical transmission spectroscopy, is presented. The chamber is made of cyclic olefin copolymers (COC), including COC X-ray windows providing ultra-low SAXS background. The design integrates a membrane insert for in situ dialysis of the 100 µl sample volume against a reservoir, which enables measurements of the same sample under multiple conditions using an in-house X-ray setup equipped with a 17.4 keV molybdenum source. The design's capabilities are demonstrated by measuring reversible structural changes in lipid and polymer systems as a function of salt concentration and pH. In the same chambers optical light transmission spectroscopy was carried out measuring the optical turbidity of the mesophases and local pH values using pH-responsive dyes. Microfluidic exchange and optical spectroscopy combined with in situ X-ray scattering enables vast applications for the study of responsive materials | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a 3D printing | |
| 650 | 4 | |a cyclic olefin copolymer | |
| 650 | 4 | |a in situ dialysis | |
| 650 | 4 | |a in-house measurements | |
| 650 | 4 | |a small-angle X-ray scattering | |
| 700 | 1 | |a Philipp, Julian |e verfasserin |4 aut | |
| 700 | 1 | |a Barkey, Martin |e verfasserin |4 aut | |
| 700 | 1 | |a Ober, Martina |e verfasserin |4 aut | |
| 700 | 1 | |a Brinkop, Achim Theo |e verfasserin |4 aut | |
| 700 | 1 | |a Simml, David |e verfasserin |4 aut | |
| 700 | 1 | |a von Westphalen, Miriam |e verfasserin |4 aut | |
| 700 | 1 | |a Nickel, Bert |e verfasserin |4 aut | |
| 700 | 1 | |a Beck, Roy |e verfasserin |4 aut | |
| 700 | 1 | |a Rädler, Joachim O |e verfasserin |4 aut | |
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