On-Site Non-enzymatic Orthogonal Activation of a Catalytic DNA Circuit for Self-Reinforced In Vivo MicroRNA Imaging
© 2022 Wiley-VCH GmbH..
The wide extracellular-intracellular distribution of microRNA requires the on-site, robust and efficient activation of catalytic DNA circuits inside live cells. Herein, we develop an efficient non-enzymatic circuitry activation strategy to realize the orthogonally controlled catalytic DNA (CCD) circuit for achieving high-fidelity in vivo microRNA imaging through multiply guaranteed molecular recognition and progressively accelerated signal amplification. For predictable on-site activation and useful catalytic efficiency, the dominating circuitry fuel strand was initially split into inactive fuel subunits that were grafted into an auxiliary catalytic circuit. There, the in-cell-specific mRNA triggered the orthogonal amplification of the active fuel strands for sensitive target detection through the chief entropy-driven catalytic DNA circuit. We believe that the on-site orthogonal circuitry activation method can contribute to clinical diagnosis and prognosis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:61 |
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| Enthalten in: |
Angewandte Chemie (International ed. in English) - 61(2022), 45 vom: 07. Nov., Seite e202206529 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
He, Shizhen [VerfasserIn] |
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| Links: |
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| Themen: |
DNA, Catalytic |
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| Anmerkungen: |
Date Completed 02.11.2022 Date Revised 06.12.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/anie.202206529 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a © 2022 Wiley-VCH GmbH. | ||
| 520 | |a The wide extracellular-intracellular distribution of microRNA requires the on-site, robust and efficient activation of catalytic DNA circuits inside live cells. Herein, we develop an efficient non-enzymatic circuitry activation strategy to realize the orthogonally controlled catalytic DNA (CCD) circuit for achieving high-fidelity in vivo microRNA imaging through multiply guaranteed molecular recognition and progressively accelerated signal amplification. For predictable on-site activation and useful catalytic efficiency, the dominating circuitry fuel strand was initially split into inactive fuel subunits that were grafted into an auxiliary catalytic circuit. There, the in-cell-specific mRNA triggered the orthogonal amplification of the active fuel strands for sensitive target detection through the chief entropy-driven catalytic DNA circuit. We believe that the on-site orthogonal circuitry activation method can contribute to clinical diagnosis and prognosis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a DNA Circuit | |
| 650 | 4 | |a Fluorescence | |
| 650 | 4 | |a RNA | |
| 650 | 4 | |a Signal Amplification | |
| 650 | 4 | |a Tumor Imaging | |
| 650 | 7 | |a DNA, Catalytic |2 NLM | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 700 | 1 | |a Yu, Shanshan |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Ruomeng |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Yingying |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Qing |e verfasserin |4 aut | |
| 700 | 1 | |a He, Yuqiu |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiaoqing |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Fuan |e verfasserin |4 aut | |
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