In vitro and in silico analysis of potential antioxidant peptides obtained from chicken hydrolysate produced using Alcalase
Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved..
Chicken hydrolysates (CHs) have been reported to protect mice against alcoholic liver injury possibly through oxidative stress reduction. In this study, the antioxidant activity of CHs was studied. Results showed that CHs exhibited significant antioxidant activity (around 600 and 400 μM TEAC/g in ORAC and ABTS assay, respectively) and could resist simulated gastrointestinal digestion. A total of 22 peptides were identified after antioxidant activity-oriented isolation using size-exclusion chromatography and high-performance liquid chromatography. Further in silico analysis and the validation of antioxidant activity revealed that novel peptides (RWGG and YYCQ) exhibited strong antioxidant activity. The most active peptide YYCQ displayed a TEAC value of 3.54 and 4.28 μM TEAC/μM in ORAC and ABTS assay, respectively. These peptides could contribute to reduce oxidative stress and protect against alcohol-induced liver injury. However, further studies understanding the bioactivity of such peptides in vivo are necessary before further applying them as functional food ingredient.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:157 |
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| Enthalten in: |
Food research international (Ottawa, Ont.) - 157(2022) vom: 28. Juli, Seite 111253 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xiao, Chuqiao [VerfasserIn] |
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| Links: |
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| Themen: |
Antioxidant |
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| Anmerkungen: |
Date Completed 29.06.2022 Date Revised 07.09.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.foodres.2022.111253 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM342825380 |
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| 520 | |a Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved. | ||
| 520 | |a Chicken hydrolysates (CHs) have been reported to protect mice against alcoholic liver injury possibly through oxidative stress reduction. In this study, the antioxidant activity of CHs was studied. Results showed that CHs exhibited significant antioxidant activity (around 600 and 400 μM TEAC/g in ORAC and ABTS assay, respectively) and could resist simulated gastrointestinal digestion. A total of 22 peptides were identified after antioxidant activity-oriented isolation using size-exclusion chromatography and high-performance liquid chromatography. Further in silico analysis and the validation of antioxidant activity revealed that novel peptides (RWGG and YYCQ) exhibited strong antioxidant activity. The most active peptide YYCQ displayed a TEAC value of 3.54 and 4.28 μM TEAC/μM in ORAC and ABTS assay, respectively. These peptides could contribute to reduce oxidative stress and protect against alcohol-induced liver injury. However, further studies understanding the bioactivity of such peptides in vivo are necessary before further applying them as functional food ingredient | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Antioxidant | |
| 650 | 4 | |a Bioactive peptides | |
| 650 | 4 | |a Chicken breast | |
| 650 | 4 | |a Mass spectrometry | |
| 650 | 4 | |a Oxidative stress | |
| 650 | 7 | |a Antioxidants |2 NLM | |
| 650 | 7 | |a Peptides |2 NLM | |
| 650 | 7 | |a Protein Hydrolysates |2 NLM | |
| 650 | 7 | |a Subtilisins |2 NLM | |
| 650 | 7 | |a EC 3.4.21.- |2 NLM | |
| 700 | 1 | |a Toldrá, Fidel |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Mouming |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Feibai |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Donghui |e verfasserin |4 aut | |
| 700 | 1 | |a Jia, Ruibo |e verfasserin |4 aut | |
| 700 | 1 | |a Mora, Leticia |e verfasserin |4 aut | |
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