Development of a Glycoform Library-based Strategy to Decipher the Role of Protein Glycosylation
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
Glycoproteins obtained from cell culture supernatants or lysates generally exist as mixtures of over 100 differently glycosylated protein forms (glycoforms). The study of glycosylation is significantly impeded because of the heterogeneous nature of glycoproteins. To overcome this challenge, we developed and optimized a glycoform library-based strategy to investigate the role of protein glycosylation. In this strategy, chemical synthesis was used to prepare individual homogeneous glycoforms and the role of glycosylation was determined by comparing a series of glycoforms with systematic differences in their glycosylation patterns.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2530 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2530(2022) vom: 27., Seite 195-211 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Yaohao [VerfasserIn] |
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| Links: |
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| Themen: |
Glycoforms |
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| Anmerkungen: |
Date Completed 29.06.2022 Date Revised 07.09.2022 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-2489-0_14 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Glycoproteins obtained from cell culture supernatants or lysates generally exist as mixtures of over 100 differently glycosylated protein forms (glycoforms). The study of glycosylation is significantly impeded because of the heterogeneous nature of glycoproteins. To overcome this challenge, we developed and optimized a glycoform library-based strategy to investigate the role of protein glycosylation. In this strategy, chemical synthesis was used to prepare individual homogeneous glycoforms and the role of glycosylation was determined by comparing a series of glycoforms with systematic differences in their glycosylation patterns | ||
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| 700 | 1 | |a Tan, Zhongping |e verfasserin |4 aut | |
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