Impact of antibiotic withdrawal and starvation conditions on plasmid elimination and consequent loss of resistance
OBJECTIVE: To assess resistance-loss due to plasmid elimination under experimental conditions, including withdrawal of antibiotics and administration of starvation conditions.
METHODS: The experimental study was conducted at the Department of Pathology, King Edward Medical University, Lahore, Pakistan, from July to December 2019. A single sensitive clinical isolate of escherichia coli, showing resistance towards ampicillin was collected and separately sub-cultured in three different culture broths: tryptic soya broth, minimal broth and control broth for a period of one month under standard laboratory conditions. Minimum inhibitory concentrations of the strains were calculated after every seven days to check antibiotic susceptibility.
RESULTS: Minimum inhibitory concentrations of the initial escherichia coli strain measured on Day 1 was 6mg/mL and it became sensitive after continual sub-culturing in the absence of antibiotics in 21 days. Due to starvation conditions, the bacterial strain exhibited sensitivity to an even lower antibiotic concentration of 1.5mg/mL on the 28th day. Bacterial growth inhibition zones determined by disc diffusion method using an ampicillin disc of 10µg/mL showed no zone of inhibition.
CONCLUSIONS: Provision of starvation conditions and withdrawal of antibiotic allowed the escherichia coli strain to exhibit gradual loss of resistance over a period of time.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:72 |
|---|---|
| Enthalten in: |
JPMA. The Journal of the Pakistan Medical Association - 72(2022), 6 vom: 23. Juni, Seite 1053-1056 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Akram, Hiba [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
7C782967RD |
|---|
| Anmerkungen: |
Date Completed 28.06.2022 Date Revised 28.06.2022 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.47391/JPMA.1209 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM342723278 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM342723278 | ||
| 003 | DE-627 | ||
| 005 | 20231226014758.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.47391/JPMA.1209 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1142.xml |
| 035 | |a (DE-627)NLM342723278 | ||
| 035 | |a (NLM)35751308 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Akram, Hiba |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Impact of antibiotic withdrawal and starvation conditions on plasmid elimination and consequent loss of resistance |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 28.06.2022 | ||
| 500 | |a Date Revised 28.06.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: To assess resistance-loss due to plasmid elimination under experimental conditions, including withdrawal of antibiotics and administration of starvation conditions | ||
| 520 | |a METHODS: The experimental study was conducted at the Department of Pathology, King Edward Medical University, Lahore, Pakistan, from July to December 2019. A single sensitive clinical isolate of escherichia coli, showing resistance towards ampicillin was collected and separately sub-cultured in three different culture broths: tryptic soya broth, minimal broth and control broth for a period of one month under standard laboratory conditions. Minimum inhibitory concentrations of the strains were calculated after every seven days to check antibiotic susceptibility | ||
| 520 | |a RESULTS: Minimum inhibitory concentrations of the initial escherichia coli strain measured on Day 1 was 6mg/mL and it became sensitive after continual sub-culturing in the absence of antibiotics in 21 days. Due to starvation conditions, the bacterial strain exhibited sensitivity to an even lower antibiotic concentration of 1.5mg/mL on the 28th day. Bacterial growth inhibition zones determined by disc diffusion method using an ampicillin disc of 10µg/mL showed no zone of inhibition | ||
| 520 | |a CONCLUSIONS: Provision of starvation conditions and withdrawal of antibiotic allowed the escherichia coli strain to exhibit gradual loss of resistance over a period of time | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Antibiotic resistance, Plasmid elimination, Antibiotic withdrawal, Resistance loss, Starvation conditions. | |
| 650 | 7 | |a Anti-Bacterial Agents |2 NLM | |
| 650 | 7 | |a Ampicillin |2 NLM | |
| 650 | 7 | |a 7C782967RD |2 NLM | |
| 700 | 1 | |a Shahid, Shaniyaal |e verfasserin |4 aut | |
| 700 | 1 | |a Bukhari, Hina |e verfasserin |4 aut | |
| 700 | 0 | |a Qurat-Ul-Ain |e verfasserin |4 aut | |
| 700 | 1 | |a Iqbal, Khadeja |e verfasserin |4 aut | |
| 700 | 1 | |a Asif, Muhammad |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t JPMA. The Journal of the Pakistan Medical Association |d 1977 |g 72(2022), 6 vom: 23. Juni, Seite 1053-1056 |w (DE-627)NLM000033170 |x 0030-9982 |7 nnns |
| 773 | 1 | 8 | |g volume:72 |g year:2022 |g number:6 |g day:23 |g month:06 |g pages:1053-1056 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.47391/JPMA.1209 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 72 |j 2022 |e 6 |b 23 |c 06 |h 1053-1056 | ||