Endotracheal tube microbiome in hospitalized patients defined largely by hospital environment
© 2022. The Author(s)..
BACKGROUND: Studies of the respiratory tract microbiome primarily focus on airway and lung microbial diversity, but it is still unclear how these microbial communities may be affected by intubation and long periods in intensive care units (ICU), an aspect that today could aid in the understanding of COVID19 progression and disease severity. This study aimed to explore and characterize the endotracheal tube (ETT) microbiome by analyzing ETT-associated microbial communities.
METHODS: This descriptive study was carried out on adult patients subjected to invasive mechanical ventilation from 2 to 21 days. ETT samples were obtained from 115 patients from ICU units in two hospitals. Bacteria isolated from endotracheal tubes belonging to the ESKAPE group were analyzed for biofilm formation using crystal violet quantification. Microbial profiles were obtained using Illumina sequencing of 16S rRNA gene.
RESULTS: The ETT microbiome was mainly composed by the phyla Proteobacteria, Firmicutes and Bacteroidetes. Microbiome composition correlated with the ICU in which patients were hospitalized, while intubation time and diagnosis of ventilator-associated pneumonia (VAP) did not show any significant association.
CONCLUSION: These results suggest that the ICU environment, or medical practices, could be a key to microbial colonization and have a direct influence on the ETT microbiomes of patients that require mechanical ventilation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:23 |
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| Enthalten in: |
Respiratory research - 23(2022), 1 vom: 24. Juni, Seite 168 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Cifuentes, Erika Alejandra [VerfasserIn] |
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| Links: |
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| Themen: |
Endotracheal tubes |
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| Anmerkungen: |
Date Completed 28.06.2022 Date Revised 16.07.2022 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1186/s12931-022-02086-7 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM342720872 |
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| 245 | 1 | 0 | |a Endotracheal tube microbiome in hospitalized patients defined largely by hospital environment |
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| 500 | |a Date Revised 16.07.2022 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2022. The Author(s). | ||
| 520 | |a BACKGROUND: Studies of the respiratory tract microbiome primarily focus on airway and lung microbial diversity, but it is still unclear how these microbial communities may be affected by intubation and long periods in intensive care units (ICU), an aspect that today could aid in the understanding of COVID19 progression and disease severity. This study aimed to explore and characterize the endotracheal tube (ETT) microbiome by analyzing ETT-associated microbial communities | ||
| 520 | |a METHODS: This descriptive study was carried out on adult patients subjected to invasive mechanical ventilation from 2 to 21 days. ETT samples were obtained from 115 patients from ICU units in two hospitals. Bacteria isolated from endotracheal tubes belonging to the ESKAPE group were analyzed for biofilm formation using crystal violet quantification. Microbial profiles were obtained using Illumina sequencing of 16S rRNA gene | ||
| 520 | |a RESULTS: The ETT microbiome was mainly composed by the phyla Proteobacteria, Firmicutes and Bacteroidetes. Microbiome composition correlated with the ICU in which patients were hospitalized, while intubation time and diagnosis of ventilator-associated pneumonia (VAP) did not show any significant association | ||
| 520 | |a CONCLUSION: These results suggest that the ICU environment, or medical practices, could be a key to microbial colonization and have a direct influence on the ETT microbiomes of patients that require mechanical ventilation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Endotracheal tubes | |
| 650 | 4 | |a Intensive care units (ICUs) | |
| 650 | 4 | |a Microbial diversity | |
| 650 | 4 | |a Respiratory tract microbiome | |
| 650 | 4 | |a Ventilator-associated pneumonia | |
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| 700 | 1 | |a Sierra, Maria A |e verfasserin |4 aut | |
| 700 | 1 | |a Yepes, Andrés Felipe |e verfasserin |4 aut | |
| 700 | 1 | |a Baldión, Ana Margarita |e verfasserin |4 aut | |
| 700 | 1 | |a Rojas, José Antonio |e verfasserin |4 aut | |
| 700 | 1 | |a Álvarez-Moreno, Carlos Arturo |e verfasserin |4 aut | |
| 700 | 1 | |a Anzola, Juan Manuel |e verfasserin |4 aut | |
| 700 | 1 | |a Zambrano, María Mercedes |e verfasserin |4 aut | |
| 700 | 1 | |a Huertas, Monica G |e verfasserin |4 aut | |
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