Faster and accurate identification of clinically important Trichosporon using MALDI TOF MS
Copyright © 2022 Indian Association of Medical Microbiologists. Published by Elsevier B.V. All rights reserved..
PURPOSE: Trichosporon species are emerging human pathogens, accounting for the second most common cause of non-candidal mycosis. Rapid and reliable identification of these agents allows a better understanding of their epidemiology and therapeutic management. The Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) technique has the potential to be precise, fast and cost-effective. However, the precision of identification totally depends upon the type of protein extraction method used and embedded database in the system. Our objectives were to standardize the protein extraction technique and expand the present Bruker database by creating an in-house database and validating it with diverse clinical Trichosporon species of Indian origin.
METHODS: Two different protein extraction protocols (on-plate and off-plate) were evaluated. The off-plate protocol was finalized for the identification. MALDI TOF MS with the existing Bruker database was evaluated for its ability to identify a total of 79 intergenic spacer 1 (IGS1) gene sequence confirmed clinical isolates of 5 different Trichosporon species.
RESULTS: As outcome, off plate protocol yielded higher accuracy (73% on the species level and 95% on the genus level) than on-plate (25% on the genus level) in terms of log scores. The existing database for Trichosporon species was enriched with 28 sequence confirmed isolates, which improved accuracy from 73% to 100% and were identified up to species level with a log score >2.3.
CONCLUSIONS: Used with standardized protein-extraction protocol along with an expanded database, MALDI-TOF MS could be a rapid and reliable approach to identify clinical Trichosporon species routinely in the laboratory.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:40 |
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| Enthalten in: |
Indian journal of medical microbiology - 40(2022), 3 vom: 03. Juli, Seite 359-364 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Parashar, Abhila [VerfasserIn] |
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| Links: |
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| Themen: |
Accurate identification |
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| Anmerkungen: |
Date Completed 16.08.2022 Date Revised 06.09.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ijmmb.2022.05.014 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 500 | |a Date Revised 06.09.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2022 Indian Association of Medical Microbiologists. Published by Elsevier B.V. All rights reserved. | ||
| 520 | |a PURPOSE: Trichosporon species are emerging human pathogens, accounting for the second most common cause of non-candidal mycosis. Rapid and reliable identification of these agents allows a better understanding of their epidemiology and therapeutic management. The Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) technique has the potential to be precise, fast and cost-effective. However, the precision of identification totally depends upon the type of protein extraction method used and embedded database in the system. Our objectives were to standardize the protein extraction technique and expand the present Bruker database by creating an in-house database and validating it with diverse clinical Trichosporon species of Indian origin | ||
| 520 | |a METHODS: Two different protein extraction protocols (on-plate and off-plate) were evaluated. The off-plate protocol was finalized for the identification. MALDI TOF MS with the existing Bruker database was evaluated for its ability to identify a total of 79 intergenic spacer 1 (IGS1) gene sequence confirmed clinical isolates of 5 different Trichosporon species | ||
| 520 | |a RESULTS: As outcome, off plate protocol yielded higher accuracy (73% on the species level and 95% on the genus level) than on-plate (25% on the genus level) in terms of log scores. The existing database for Trichosporon species was enriched with 28 sequence confirmed isolates, which improved accuracy from 73% to 100% and were identified up to species level with a log score >2.3 | ||
| 520 | |a CONCLUSIONS: Used with standardized protein-extraction protocol along with an expanded database, MALDI-TOF MS could be a rapid and reliable approach to identify clinical Trichosporon species routinely in the laboratory | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Accurate identification | |
| 650 | 4 | |a MALDI TOF | |
| 650 | 4 | |a Mass spectrometry | |
| 650 | 4 | |a Protein extraction protocol | |
| 650 | 4 | |a Trichosporon | |
| 700 | 1 | |a Rastogi, Vijaylatha |e verfasserin |4 aut | |
| 700 | 1 | |a Rudramurthy, Shivaprakash M |e verfasserin |4 aut | |
| 700 | 1 | |a Ghosh, Anup Kumar |e verfasserin |4 aut | |
| 700 | 1 | |a Chander, Jagdish |e verfasserin |4 aut | |
| 700 | 1 | |a Kindo, Anupma Jyoti |e verfasserin |4 aut | |
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