Outbreak of IncX8 Plasmid-Mediated KPC-3-Producing Enterobacterales Infection, China
Carbapenem-resistant Enterobacterales (CRE) infection is highly endemic in China; Klebsiella pneumoniae carbapenemase (KPC) 2-producing CRE is the most common, whereas KPC-3-producing CRE is rare. We report an outbreak of KPC-3-producing Enterobacterales infection in China. During August 2020-June 2021, 25 blaKPC-3-positive Enterobacteriale isolates were detected from 24 patients in China. Whole-genome sequencing analysis revealed that the blaKPC-3 genes were harbored by IncX8 plasmids. The outbreak involved clonal expansion of KPC-3-producing Serratia marcescens and transmission of blaKPC-3 plasmids across different species. The blaKPC-3 plasmids demonstrated high conjugation frequencies (10-3 to 10-4). A Galleria mellonella infection model showed that 2 sequence type 65 K2 K. pneumoniae strains containing blaKPC-3 plasmids were highly virulent. A ceftazidime/avibactam in vitro selection assay indicated that the KPC-3-producing strains can readily develop resistance. The spread of blaKPC-3-harboring IncX8 plasmids and these KPC-3 strains should be closely monitored in China and globally.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:28 |
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| Enthalten in: |
Emerging infectious diseases - 28(2022), 7 vom: 12. Juli, Seite 1421-1430 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chen, Lan [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 24.06.2022 Date Revised 16.07.2022 published: Print Citation Status MEDLINE |
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| doi: |
10.3201/eid2807.212181 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM342523287 |
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| 520 | |a Carbapenem-resistant Enterobacterales (CRE) infection is highly endemic in China; Klebsiella pneumoniae carbapenemase (KPC) 2-producing CRE is the most common, whereas KPC-3-producing CRE is rare. We report an outbreak of KPC-3-producing Enterobacterales infection in China. During August 2020-June 2021, 25 blaKPC-3-positive Enterobacteriale isolates were detected from 24 patients in China. Whole-genome sequencing analysis revealed that the blaKPC-3 genes were harbored by IncX8 plasmids. The outbreak involved clonal expansion of KPC-3-producing Serratia marcescens and transmission of blaKPC-3 plasmids across different species. The blaKPC-3 plasmids demonstrated high conjugation frequencies (10-3 to 10-4). A Galleria mellonella infection model showed that 2 sequence type 65 K2 K. pneumoniae strains containing blaKPC-3 plasmids were highly virulent. A ceftazidime/avibactam in vitro selection assay indicated that the KPC-3-producing strains can readily develop resistance. The spread of blaKPC-3-harboring IncX8 plasmids and these KPC-3 strains should be closely monitored in China and globally | ||
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| 700 | 1 | |a Zhou, Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Chunyang |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Yinjuan |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Xiaocui |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Bingjie |e verfasserin |4 aut | |
| 700 | 1 | |a Rao, Lulin |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Yanlei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jiao |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Liang |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Fangyou |e verfasserin |4 aut | |
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