Alterations in B Cell and Follicular T-Helper Cell Subsets in Patients with Acute COVID-19 and COVID-19 Convalescents
BACKGROUND: Humoral immunity requires interaction between B cell and T follicular helper cells (Tfh) to produce effective immune response, but the data regarding a role of B cells and Tfh in SARS-CoV-2 defense are still sparse.
METHODS: Blood samples from patients with acute COVID-19 (n = 64), convalescents patients who had specific IgG to SARS-CoV-2 N-protein (n = 55), and healthy donors with no detectable antibodies to any SARS-CoV-2 proteins (HC, n = 44) were analyses by multicolor flow cytometry.
RESULTS: Patients with acute COVID-19 showed decreased levels of memory B cells subsets and increased proportion plasma cell precursors compared to HC and COVID-19 convalescent patients, whereas for the latter the elevated numbers of virgin naïve, Bm2' and "Bm3+Bm4" was found if compared with HC. During acute COVID-19 CXCR3+CCR6- Tfh1-like cells were decreased and the levels of CXCR3-CCR6+ Tfh17-like were increased then in HC and convalescent patients. Finally, COVID-19 convalescent patients had increased levels of Tfh2-, Tfh17- and DP Tfh-like cells while comparing their amount with HC.
CONCLUSIONS: Our data indicate that COVID-19 can impact the humoral immunity in the long-term.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:44 |
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| Enthalten in: |
Current issues in molecular biology - 44(2021), 1 vom: 30. Dez., Seite 194-205 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kudryavtsev, Igor V [VerfasserIn] |
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| Links: |
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| Themen: |
B cell subsets |
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| Anmerkungen: |
Date Revised 16.07.2022 published: Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.3390/cimb44010014 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM34244655X |
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| 100 | 1 | |a Kudryavtsev, Igor V |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Alterations in B Cell and Follicular T-Helper Cell Subsets in Patients with Acute COVID-19 and COVID-19 Convalescents |
| 264 | 1 | |c 2021 | |
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| 500 | |a Date Revised 16.07.2022 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a BACKGROUND: Humoral immunity requires interaction between B cell and T follicular helper cells (Tfh) to produce effective immune response, but the data regarding a role of B cells and Tfh in SARS-CoV-2 defense are still sparse | ||
| 520 | |a METHODS: Blood samples from patients with acute COVID-19 (n = 64), convalescents patients who had specific IgG to SARS-CoV-2 N-protein (n = 55), and healthy donors with no detectable antibodies to any SARS-CoV-2 proteins (HC, n = 44) were analyses by multicolor flow cytometry | ||
| 520 | |a RESULTS: Patients with acute COVID-19 showed decreased levels of memory B cells subsets and increased proportion plasma cell precursors compared to HC and COVID-19 convalescent patients, whereas for the latter the elevated numbers of virgin naïve, Bm2' and "Bm3+Bm4" was found if compared with HC. During acute COVID-19 CXCR3+CCR6- Tfh1-like cells were decreased and the levels of CXCR3-CCR6+ Tfh17-like were increased then in HC and convalescent patients. Finally, COVID-19 convalescent patients had increased levels of Tfh2-, Tfh17- and DP Tfh-like cells while comparing their amount with HC | ||
| 520 | |a CONCLUSIONS: Our data indicate that COVID-19 can impact the humoral immunity in the long-term | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a B cell subsets | |
| 650 | 4 | |a COVID-19 | |
| 650 | 4 | |a SARS-CoV-2 | |
| 650 | 4 | |a T follicular helper cells | |
| 650 | 4 | |a Tfh cell subsets | |
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| 700 | 1 | |a Arsentieva, Natalia A |e verfasserin |4 aut | |
| 700 | 1 | |a Batsunov, Oleg K |e verfasserin |4 aut | |
| 700 | 1 | |a Korobova, Zoia R |e verfasserin |4 aut | |
| 700 | 1 | |a Khamitova, Irina V |e verfasserin |4 aut | |
| 700 | 1 | |a Isakov, Dmitrii V |e verfasserin |4 aut | |
| 700 | 1 | |a Kuznetsova, Raisa N |e verfasserin |4 aut | |
| 700 | 1 | |a Rubinstein, Artem A |e verfasserin |4 aut | |
| 700 | 1 | |a Stanevich, Oksana V |e verfasserin |4 aut | |
| 700 | 1 | |a Lebedeva, Aleksandra A |e verfasserin |4 aut | |
| 700 | 1 | |a Vorobyov, Evgeny A |e verfasserin |4 aut | |
| 700 | 1 | |a Vorobyova, Snejana V |e verfasserin |4 aut | |
| 700 | 1 | |a Kulikov, Alexander N |e verfasserin |4 aut | |
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