RNA Sequencing Unveils Very Small RNAs With Potential Regulatory Functions in Bacteria
Copyright © 2022 Diallo, Ho, Lalaouna, Massé and Provost..
RNA sequencing (RNA-seq) is the gold standard for the discovery of small non-coding RNAs. Following a long-standing approach, reads shorter than 16 nucleotides (nt) are removed from the small RNA sequencing libraries or datasets. The serendipitous discovery of an eukaryotic 12 nt-long RNA species capable of modulating the microRNA from which they derive prompted us to challenge this dogma and, by expanding the window of RNA sizes down to 8 nt, to confirm the existence of functional very small RNAs (vsRNAs <16 nt). Here we report the detailed profiling of vsRNAs in Escherichia coli, E. coli-derived outer membrane vesicles (OMVs) and five other bacterial strains (Pseudomonas aeruginosa PA7, P. aeruginosa PAO1, Salmonella enterica serovar Typhimurium 14028S, Legionella pneumophila JR32 Philadelphia-1 and Staphylococcus aureus HG001). vsRNAs of 8-15 nt in length [RNAs (8-15 nt)] were found to be more abundant than RNAs of 16-30 nt in length [RNAs (16-30 nt)]. vsRNA biotypes were distinct and varied within and across bacterial species and accounted for one third of reads identified in the 8-30 nt window. The tRNA-derived fragments (tRFs) have appeared as a major biotype among the vsRNAs, notably Ile-tRF and Ala-tRF, and were selectively loaded in OMVs. tRF-derived vsRNAs appear to be thermodynamically stable with at least 2 G-C basepairs and stem-loop structure. The analyzed tRF-derived vsRNAs are predicted to target several human host mRNAs with diverse functions. Bacterial vsRNAs and OMV-derived vsRNAs could be novel players likely modulating the intricate relationship between pathogens and their hosts.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2022 |
---|---|
Erschienen: |
2022 |
Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
---|---|
Enthalten in: |
Frontiers in molecular biosciences - 9(2022) vom: 16., Seite 914991 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Diallo, Idrissa [VerfasserIn] |
---|
Links: |
---|
Themen: |
Bacteria |
---|
Anmerkungen: |
Date Revised 16.07.2022 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
---|
doi: |
10.3389/fmolb.2022.914991 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM342413821 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM342413821 | ||
003 | DE-627 | ||
005 | 20231226014048.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231226s2022 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.3389/fmolb.2022.914991 |2 doi | |
028 | 5 | 2 | |a pubmed24n1141.xml |
035 | |a (DE-627)NLM342413821 | ||
035 | |a (NLM)35720117 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Diallo, Idrissa |e verfasserin |4 aut | |
245 | 1 | 0 | |a RNA Sequencing Unveils Very Small RNAs With Potential Regulatory Functions in Bacteria |
264 | 1 | |c 2022 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Revised 16.07.2022 | ||
500 | |a published: Electronic-eCollection | ||
500 | |a Citation Status PubMed-not-MEDLINE | ||
520 | |a Copyright © 2022 Diallo, Ho, Lalaouna, Massé and Provost. | ||
520 | |a RNA sequencing (RNA-seq) is the gold standard for the discovery of small non-coding RNAs. Following a long-standing approach, reads shorter than 16 nucleotides (nt) are removed from the small RNA sequencing libraries or datasets. The serendipitous discovery of an eukaryotic 12 nt-long RNA species capable of modulating the microRNA from which they derive prompted us to challenge this dogma and, by expanding the window of RNA sizes down to 8 nt, to confirm the existence of functional very small RNAs (vsRNAs <16 nt). Here we report the detailed profiling of vsRNAs in Escherichia coli, E. coli-derived outer membrane vesicles (OMVs) and five other bacterial strains (Pseudomonas aeruginosa PA7, P. aeruginosa PAO1, Salmonella enterica serovar Typhimurium 14028S, Legionella pneumophila JR32 Philadelphia-1 and Staphylococcus aureus HG001). vsRNAs of 8-15 nt in length [RNAs (8-15 nt)] were found to be more abundant than RNAs of 16-30 nt in length [RNAs (16-30 nt)]. vsRNA biotypes were distinct and varied within and across bacterial species and accounted for one third of reads identified in the 8-30 nt window. The tRNA-derived fragments (tRFs) have appeared as a major biotype among the vsRNAs, notably Ile-tRF and Ala-tRF, and were selectively loaded in OMVs. tRF-derived vsRNAs appear to be thermodynamically stable with at least 2 G-C basepairs and stem-loop structure. The analyzed tRF-derived vsRNAs are predicted to target several human host mRNAs with diverse functions. Bacterial vsRNAs and OMV-derived vsRNAs could be novel players likely modulating the intricate relationship between pathogens and their hosts | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a E. coli | |
650 | 4 | |a RNA sequencing | |
650 | 4 | |a bacteria | |
650 | 4 | |a outer membrane vesicle (OMV) | |
650 | 4 | |a tRNA fragment (tRF) | |
650 | 4 | |a very small RNA (vsRNA) | |
700 | 1 | |a Ho, Jeffrey |e verfasserin |4 aut | |
700 | 1 | |a Lalaouna, David |e verfasserin |4 aut | |
700 | 1 | |a Massé, Eric |e verfasserin |4 aut | |
700 | 1 | |a Provost, Patrick |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Frontiers in molecular biosciences |d 2014 |g 9(2022) vom: 16., Seite 914991 |w (DE-627)NLM249149435 |x 2296-889X |7 nnns |
773 | 1 | 8 | |g volume:9 |g year:2022 |g day:16 |g pages:914991 |
856 | 4 | 0 | |u http://dx.doi.org/10.3389/fmolb.2022.914991 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 9 |j 2022 |b 16 |h 914991 |