A global lipid map reveals host dependency factors conserved across SARS-CoV-2 variants
© 2022. The Author(s)..
A comprehensive understanding of host dependency factors for SARS-CoV-2 remains elusive. Here, we map alterations in host lipids following SARS-CoV-2 infection using nontargeted lipidomics. We find that SARS-CoV-2 rewires host lipid metabolism, significantly altering hundreds of lipid species to effectively establish infection. We correlate these changes with viral protein activity by transfecting human cells with each viral protein and performing lipidomics. We find that lipid droplet plasticity is a key feature of infection and that viral propagation can be blocked by small-molecule glycerolipid biosynthesis inhibitors. We find that this inhibition was effective against the main variants of concern (alpha, beta, gamma, and delta), indicating that glycerolipid biosynthesis is a conserved host dependency factor that supports this evolving virus.
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Nature communications - 13(2022), 1 vom: 17. Juni, Seite 3487 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Farley, Scotland E [VerfasserIn] |
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Journal Article |
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| Anmerkungen: |
Date Completed 21.06.2022 Date Revised 31.08.2023 published: Electronic UpdateOf: bioRxiv. 2022 Feb 15;:. - PMID 35194611 Citation Status MEDLINE |
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| doi: |
10.1038/s41467-022-31097-7 |
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| PPN (Katalog-ID): |
NLM342366831 |
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| 245 | 1 | 2 | |a A global lipid map reveals host dependency factors conserved across SARS-CoV-2 variants |
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| 500 | |a published: Electronic | ||
| 500 | |a UpdateOf: bioRxiv. 2022 Feb 15;:. - PMID 35194611 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2022. The Author(s). | ||
| 520 | |a A comprehensive understanding of host dependency factors for SARS-CoV-2 remains elusive. Here, we map alterations in host lipids following SARS-CoV-2 infection using nontargeted lipidomics. We find that SARS-CoV-2 rewires host lipid metabolism, significantly altering hundreds of lipid species to effectively establish infection. We correlate these changes with viral protein activity by transfecting human cells with each viral protein and performing lipidomics. We find that lipid droplet plasticity is a key feature of infection and that viral propagation can be blocked by small-molecule glycerolipid biosynthesis inhibitors. We find that this inhibition was effective against the main variants of concern (alpha, beta, gamma, and delta), indicating that glycerolipid biosynthesis is a conserved host dependency factor that supports this evolving virus | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
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| 650 | 7 | |a Lipids |2 NLM | |
| 650 | 7 | |a Viral Proteins |2 NLM | |
| 700 | 1 | |a Kyle, Jennifer E |e verfasserin |4 aut | |
| 700 | 1 | |a Leier, Hans C |e verfasserin |4 aut | |
| 700 | 1 | |a Bramer, Lisa M |e verfasserin |4 aut | |
| 700 | 1 | |a Weinstein, Jules B |e verfasserin |4 aut | |
| 700 | 1 | |a Bates, Timothy A |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Joon-Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Metz, Thomas O |e verfasserin |4 aut | |
| 700 | 1 | |a Schultz, Carsten |e verfasserin |4 aut | |
| 700 | 1 | |a Tafesse, Fikadu G |e verfasserin |4 aut | |
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