Application of digital PCR to determine the reliability of Xpert Xpress SARS-CoV-2 assay with envelope (E) gene negative and nucleocapsid (N2) gene positive results
Copyright © 2022 Elsevier Inc. All rights reserved..
This study used digital polymerase chain reaction (dPCR) to determine whether envelope (E) gene-negative and nucleocapsid (N2) gene-positive (E-N+) results obtained with the Cepheid Xpert Xpress SARS-CoV-2 assay are reliable. Using droplet digital PCR results as a reference, 18 of 22 E-N+ samples with a low viral load (81.8%) were identified as true positives.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:103 |
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| Enthalten in: |
Diagnostic microbiology and infectious disease - 103(2022), 4 vom: 05. Aug., Seite 115726 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wong, River Chun-Wai [VerfasserIn] |
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| Links: |
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| Themen: |
Cepheid Xpert Xpress SARS-CoV-2 assay |
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| Anmerkungen: |
Date Completed 12.07.2022 Date Revised 21.12.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.diagmicrobio.2022.115726 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM342127551 |
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| 520 | |a This study used digital polymerase chain reaction (dPCR) to determine whether envelope (E) gene-negative and nucleocapsid (N2) gene-positive (E-N+) results obtained with the Cepheid Xpert Xpress SARS-CoV-2 assay are reliable. Using droplet digital PCR results as a reference, 18 of 22 E-N+ samples with a low viral load (81.8%) were identified as true positives | ||
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| 700 | 1 | |a Lee, Lam-Kwong |e verfasserin |4 aut | |
| 700 | 1 | |a Leung, Eddie Chi-Man |e verfasserin |4 aut | |
| 700 | 1 | |a Lai, Raymond Wai-Man |e verfasserin |4 aut | |
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