Precise control of microtubule disassembly in living cells
© 2022 The Authors. Published under the terms of the CC BY NC ND 4.0 license..
Microtubules tightly regulate various cellular activities. Our understanding of microtubules is largely based on experiments using microtubule-targeting agents, which, however, are insufficient to dissect the dynamic mechanisms of specific microtubule populations, due to their slow effects on the entire pool of microtubules. To overcome this technological limitation, we have used chemo and optogenetics to disassemble specific microtubule subtypes, including tyrosinated microtubules, primary cilia, mitotic spindles, and intercellular bridges, by rapidly recruiting engineered microtubule-cleaving enzymes onto target microtubules in a reversible manner. Using this approach, we show that acute microtubule disassembly swiftly halts vesicular trafficking and lysosomal dynamics. It also immediately triggers Golgi and ER reorganization and slows the fusion/fission of mitochondria without affecting mitochondrial membrane potential. In addition, cell rigidity is increased after microtubule disruption owing to increased contractile stress fibers. Microtubule disruption furthermore prevents cell division, but does not cause cell death during interphase. Overall, the reported tools facilitate detailed analysis of how microtubules precisely regulate cellular architecture and functions.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:41 |
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| Enthalten in: |
The EMBO journal - 41(2022), 15 vom: 01. Aug., Seite e110472 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Grace Y [VerfasserIn] |
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| Links: |
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| Themen: |
Intercellular bridge |
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| Anmerkungen: |
Date Completed 02.08.2022 Date Revised 07.09.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.15252/embj.2021110472 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM342083171 |
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| 520 | |a © 2022 The Authors. Published under the terms of the CC BY NC ND 4.0 license. | ||
| 520 | |a Microtubules tightly regulate various cellular activities. Our understanding of microtubules is largely based on experiments using microtubule-targeting agents, which, however, are insufficient to dissect the dynamic mechanisms of specific microtubule populations, due to their slow effects on the entire pool of microtubules. To overcome this technological limitation, we have used chemo and optogenetics to disassemble specific microtubule subtypes, including tyrosinated microtubules, primary cilia, mitotic spindles, and intercellular bridges, by rapidly recruiting engineered microtubule-cleaving enzymes onto target microtubules in a reversible manner. Using this approach, we show that acute microtubule disassembly swiftly halts vesicular trafficking and lysosomal dynamics. It also immediately triggers Golgi and ER reorganization and slows the fusion/fission of mitochondria without affecting mitochondrial membrane potential. In addition, cell rigidity is increased after microtubule disruption owing to increased contractile stress fibers. Microtubule disruption furthermore prevents cell division, but does not cause cell death during interphase. Overall, the reported tools facilitate detailed analysis of how microtubules precisely regulate cellular architecture and functions | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a intercellular bridge | |
| 650 | 4 | |a microtubule | |
| 650 | 4 | |a mitotic spindle | |
| 650 | 4 | |a primary cilium | |
| 650 | 4 | |a spastin | |
| 700 | 1 | |a Chen, Shiau-Chi |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Gang-Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Shaiv, Kritika |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Pin-Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Hsuan |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, Shi-Rong |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Wen-Ting |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Shih-Han |e verfasserin |4 aut | |
| 700 | 1 | |a Chang, Ya-Chu |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Hsien-Chu |e verfasserin |4 aut | |
| 700 | 1 | |a Kao, Ching-Lin |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Pin-Chiao |e verfasserin |4 aut | |
| 700 | 1 | |a Chao, Ming-Hong |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Yian-Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Ming-Jer |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Yu-Chun |e verfasserin |4 aut | |
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