MiR-199a-3p in mouse bone marrow mesenchymal stem cell exosomes increases epithelial sodium channel expression in lung injury
© 2022 Société Française de Pharmacologie et de Thérapeutique. Published by John Wiley & Sons Ltd..
Acute lung injury (ALI) causes significant morbidity and mortality in critically ill patients, which often presents with extensive accumulation of activated inflammatory cells and diffused alveolar damage accompanied by oxidative stress. Exosomes are nanovesicles, which have notable anti-inflammatory and repair properties, thus alleviating the symptoms of ALI. Epithelial sodium channel (ENaC) is essential for the transepithelial absorption of Na+ and fluid from alveolar spaces. We studied the effects of bone marrow mesenchymal stem cell exosomes (BMSC-exo) on the apoptosis and protein expression of ENaC in primary mouse alveolar epithelial type 2 cells (AT 2 cells). Moreover, the change of miR-199a-3p in AT 2 cells was detected by qRT-PCR, and we studied the regulation of miR-199a-3p on ENaC protein expression. Our results demonstrated that BMSC-exo could not only improve viability and reduce apoptosis in AT 2 cells, but also enhance the expression of ENaC protein and miR-199a-3p. Meanwhile, the upregulation of miR-199a-3p resulted in increased expression of ENaC protein. In summary, the BMSC-exo could participate in the regulation of ENaC through miR-199a-3p originated from BMSC-exo, thereby providing a new pharmacological tool for the treatment of ALI.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:36 |
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| Enthalten in: |
Fundamental & clinical pharmacology - 36(2022), 6 vom: 03. Dez., Seite 1011-1019 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chen, Lei [VerfasserIn] |
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| Links: |
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| Themen: |
Acute lung injury |
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| Anmerkungen: |
Date Completed 15.11.2022 Date Revised 15.11.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/fcp.12807 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM341897094 |
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| 520 | |a © 2022 Société Française de Pharmacologie et de Thérapeutique. Published by John Wiley & Sons Ltd. | ||
| 520 | |a Acute lung injury (ALI) causes significant morbidity and mortality in critically ill patients, which often presents with extensive accumulation of activated inflammatory cells and diffused alveolar damage accompanied by oxidative stress. Exosomes are nanovesicles, which have notable anti-inflammatory and repair properties, thus alleviating the symptoms of ALI. Epithelial sodium channel (ENaC) is essential for the transepithelial absorption of Na+ and fluid from alveolar spaces. We studied the effects of bone marrow mesenchymal stem cell exosomes (BMSC-exo) on the apoptosis and protein expression of ENaC in primary mouse alveolar epithelial type 2 cells (AT 2 cells). Moreover, the change of miR-199a-3p in AT 2 cells was detected by qRT-PCR, and we studied the regulation of miR-199a-3p on ENaC protein expression. Our results demonstrated that BMSC-exo could not only improve viability and reduce apoptosis in AT 2 cells, but also enhance the expression of ENaC protein and miR-199a-3p. Meanwhile, the upregulation of miR-199a-3p resulted in increased expression of ENaC protein. In summary, the BMSC-exo could participate in the regulation of ENaC through miR-199a-3p originated from BMSC-exo, thereby providing a new pharmacological tool for the treatment of ALI | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a acute lung injury | |
| 650 | 4 | |a bone marrow mesenchymal stem cells | |
| 650 | 4 | |a epithelial sodium channel | |
| 650 | 4 | |a exosomes | |
| 650 | 4 | |a miR-199a-3p | |
| 650 | 7 | |a Epithelial Sodium Channels |2 NLM | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 650 | 7 | |a Mirn199 microRNA, mouse |2 NLM | |
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| 700 | 1 | |a Du, Danni |e verfasserin |4 aut | |
| 700 | 1 | |a Cui, Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Nie, Hongguang |e verfasserin |4 aut | |
| 700 | 1 | |a Ding, Yan |e verfasserin |4 aut | |
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