Quantitative Site-Specific Chemoproteomic Profiling of Protein Lipoylation
Protein lipoylation is an evolutionarily conserved post-translational modification from prokaryotes to eukaryotes. Lipoylation is implicated with several human diseases, including metabolic disorders, cancer, and Alzheimer's disease. While individual lipoylated proteins have been biochemically studied, a strategy for globally quantifying lipoylation with site-specific resolution in proteomes is still lacking. Herein, we developed a butyraldehyde-alkynyl probe to specifically label and enrich lipoylations in complexed biological samples. Combined with a chemoproteomic pipeline using customized tandem enzyme digestions and a biotin enrichment tag with enhanced ionization, we successfully quantified all known lipoylation sites in both Escherichia coli (E. coli) and human proteomes. The strategy enabled us to dissect the dependence of three evolutionarily related lipoylation sites in dihydrolipoamide acetyltransferase (ODP2) in E. coli and evaluated the functional connection between the de novo lipoylation synthetic pathway and the salvage pathway. Our chemoproteomic platform provides a useful tool to monitor the state of lipoylation in proteome samples, which will help decipher molecular mechanisms of lipoylation-related diseases.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2022 |
---|---|
Erschienen: |
2022 |
Enthalten in: |
Zur Gesamtaufnahme - volume:144 |
---|---|
Enthalten in: |
Journal of the American Chemical Society - 144(2022), 23 vom: 15. Juni, Seite 10320-10329 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Lai, Shuchang [VerfasserIn] |
---|
Links: |
---|
Themen: |
---|
Anmerkungen: |
Date Completed 16.06.2022 Date Revised 19.07.2022 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1021/jacs.2c01528 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM341704660 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM341704660 | ||
003 | DE-627 | ||
005 | 20231226012401.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231226s2022 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1021/jacs.2c01528 |2 doi | |
028 | 5 | 2 | |a pubmed24n1138.xml |
035 | |a (DE-627)NLM341704660 | ||
035 | |a (NLM)35648456 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Lai, Shuchang |e verfasserin |4 aut | |
245 | 1 | 0 | |a Quantitative Site-Specific Chemoproteomic Profiling of Protein Lipoylation |
264 | 1 | |c 2022 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 16.06.2022 | ||
500 | |a Date Revised 19.07.2022 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Protein lipoylation is an evolutionarily conserved post-translational modification from prokaryotes to eukaryotes. Lipoylation is implicated with several human diseases, including metabolic disorders, cancer, and Alzheimer's disease. While individual lipoylated proteins have been biochemically studied, a strategy for globally quantifying lipoylation with site-specific resolution in proteomes is still lacking. Herein, we developed a butyraldehyde-alkynyl probe to specifically label and enrich lipoylations in complexed biological samples. Combined with a chemoproteomic pipeline using customized tandem enzyme digestions and a biotin enrichment tag with enhanced ionization, we successfully quantified all known lipoylation sites in both Escherichia coli (E. coli) and human proteomes. The strategy enabled us to dissect the dependence of three evolutionarily related lipoylation sites in dihydrolipoamide acetyltransferase (ODP2) in E. coli and evaluated the functional connection between the de novo lipoylation synthetic pathway and the salvage pathway. Our chemoproteomic platform provides a useful tool to monitor the state of lipoylation in proteome samples, which will help decipher molecular mechanisms of lipoylation-related diseases | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 7 | |a Proteome |2 NLM | |
700 | 1 | |a Chen, Ying |e verfasserin |4 aut | |
700 | 1 | |a Yang, Fan |e verfasserin |4 aut | |
700 | 1 | |a Xiao, Weidi |e verfasserin |4 aut | |
700 | 1 | |a Liu, Yuan |e verfasserin |4 aut | |
700 | 1 | |a Wang, Chu |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Journal of the American Chemical Society |d 1945 |g 144(2022), 23 vom: 15. Juni, Seite 10320-10329 |w (DE-627)NLM00000569X |x 1520-5126 |7 nnns |
773 | 1 | 8 | |g volume:144 |g year:2022 |g number:23 |g day:15 |g month:06 |g pages:10320-10329 |
856 | 4 | 0 | |u http://dx.doi.org/10.1021/jacs.2c01528 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 144 |j 2022 |e 23 |b 15 |c 06 |h 10320-10329 |