A Distinct Dexamethasone-Dependent Gene Expression Profile in the Lungs of COVID-19 Patients
© The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com..
The effects of dexamethasone (DXM) treatment on pulmonary immunity in COVID-19-associated acute respiratory distress syndrome (CARDS) remain insufficiently understood. We performed transcriptomic RNA-seq analysis of bronchoalveolar lavage fluid from 20 mechanically ventilated patients: 12 with CARDS (with or without DXM) and 8 non-COVID-19 critically ill controls. CARDS with DXM was characterized by upregulation of genes related to B-cell and complement pathway activation, antigen presentation, phagocytosis, and FC-γ receptor signaling. Most interferon-stimulated genes were upregulated in CARDS, particularly in CARDS without DXM. In conclusion, DXM treatment was not associated with regulation of proinflammatory pathways in CARDS but with regulation of other local immune responses. Clinical Trials Registration. NCT04354584.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:226 |
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| Enthalten in: |
The Journal of infectious diseases - 226(2022), 12 vom: 13. Dez., Seite 2137-2141 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Fahnøe, Ulrik [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 20.12.2022 Date Revised 03.01.2023 published: Print ClinicalTrials.gov: NCT04354584 Citation Status MEDLINE |
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| doi: |
10.1093/infdis/jiac218 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM341621064 |
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| 100 | 1 | |a Fahnøe, Ulrik |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A Distinct Dexamethasone-Dependent Gene Expression Profile in the Lungs of COVID-19 Patients |
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| 500 | |a ClinicalTrials.gov: NCT04354584 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com. | ||
| 520 | |a The effects of dexamethasone (DXM) treatment on pulmonary immunity in COVID-19-associated acute respiratory distress syndrome (CARDS) remain insufficiently understood. We performed transcriptomic RNA-seq analysis of bronchoalveolar lavage fluid from 20 mechanically ventilated patients: 12 with CARDS (with or without DXM) and 8 non-COVID-19 critically ill controls. CARDS with DXM was characterized by upregulation of genes related to B-cell and complement pathway activation, antigen presentation, phagocytosis, and FC-γ receptor signaling. Most interferon-stimulated genes were upregulated in CARDS, particularly in CARDS without DXM. In conclusion, DXM treatment was not associated with regulation of proinflammatory pathways in CARDS but with regulation of other local immune responses. Clinical Trials Registration. NCT04354584 | ||
| 650 | 4 | |a Clinical Trial | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a acute respiratory distress syndrome | |
| 650 | 4 | |a bronchoalveolar lavage fluid | |
| 650 | 4 | |a coronavirus disease 2019 | |
| 650 | 4 | |a inflammation | |
| 650 | 4 | |a interferon stimulated genes | |
| 650 | 4 | |a transcriptome profiling | |
| 650 | 7 | |a Dexamethasone |2 NLM | |
| 650 | 7 | |a 7S5I7G3JQL |2 NLM | |
| 700 | 1 | |a Ronit, Andreas |e verfasserin |4 aut | |
| 700 | 1 | |a Berg, Ronan M G |e verfasserin |4 aut | |
| 700 | 1 | |a Jørgensen, Sofie E |e verfasserin |4 aut | |
| 700 | 1 | |a Mogensen, Trine H |e verfasserin |4 aut | |
| 700 | 1 | |a Underwood, Alexander P |e verfasserin |4 aut | |
| 700 | 1 | |a Scheel, Troels K H |e verfasserin |4 aut | |
| 700 | 1 | |a Bukh, Jens |e verfasserin |4 aut | |
| 700 | 1 | |a Plovsing, Ronni R |e verfasserin |4 aut | |
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