A genetic engineering strategy for editing near-infrared-II fluorophores
© 2022. The Author(s)..
The second near-infrared (NIR-II) window is a fundamental modality for deep-tissue in vivo imaging. However, it is challenging to synthesize NIR-II probes with high quantum yields (QYs), good biocompatibility, satisfactory pharmacokinetics, and tunable biological properties. Conventional long-wavelength probes, such as inorganic probes (which often contain heavy metal atoms in their scaffolds) and organic dyes (which contain large π-conjugated groups), exhibit poor biosafety, low QYs, and/or uncontrollable pharmacokinetic properties. Herein, we present a bioengineering strategy that can replace the conventional chemical synthesis methods for generating NIR-II contrast agents. We use a genetic engineering technique to obtain a series of albumin fragments and recombinant proteins containing one or multiple domains that form covalent bonds with chloro-containing cyanine dyes. These albumin variants protect the inserted dyes and remarkably enhance their brightness. The albumin variants can also be genetically edited to develop size-tunable complexes with precisely tailored pharmacokinetics. The proteins can also be conjugated to biofunctional molecules without impacting the complexed dyes. This combination of albumin mutants and clinically-used cyanine dyes can help widen the clinical application prospects of NIR-II fluorophores.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Nature communications - 13(2022), 1 vom: 23. Mai, Seite 2853 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Tian, Rui [VerfasserIn] |
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| Links: |
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| Themen: |
Albumins |
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| Anmerkungen: |
Date Completed 25.05.2022 Date Revised 16.07.2022 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41467-022-30304-9 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM341288470 |
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| 520 | |a © 2022. The Author(s). | ||
| 520 | |a The second near-infrared (NIR-II) window is a fundamental modality for deep-tissue in vivo imaging. However, it is challenging to synthesize NIR-II probes with high quantum yields (QYs), good biocompatibility, satisfactory pharmacokinetics, and tunable biological properties. Conventional long-wavelength probes, such as inorganic probes (which often contain heavy metal atoms in their scaffolds) and organic dyes (which contain large π-conjugated groups), exhibit poor biosafety, low QYs, and/or uncontrollable pharmacokinetic properties. Herein, we present a bioengineering strategy that can replace the conventional chemical synthesis methods for generating NIR-II contrast agents. We use a genetic engineering technique to obtain a series of albumin fragments and recombinant proteins containing one or multiple domains that form covalent bonds with chloro-containing cyanine dyes. These albumin variants protect the inserted dyes and remarkably enhance their brightness. The albumin variants can also be genetically edited to develop size-tunable complexes with precisely tailored pharmacokinetics. The proteins can also be conjugated to biofunctional molecules without impacting the complexed dyes. This combination of albumin mutants and clinically-used cyanine dyes can help widen the clinical application prospects of NIR-II fluorophores | ||
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| 650 | 7 | |a Fluorescent Dyes |2 NLM | |
| 650 | 7 | |a Ionophores |2 NLM | |
| 650 | 7 | |a Quinolines |2 NLM | |
| 700 | 1 | |a Feng, Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Wei, Long |e verfasserin |4 aut | |
| 700 | 1 | |a Dai, Daoguo |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Pan, Haifeng |e verfasserin |4 aut | |
| 700 | 1 | |a Ge, Shengxiang |e verfasserin |4 aut | |
| 700 | 1 | |a Bai, Lang |e verfasserin |4 aut | |
| 700 | 1 | |a Ke, Chaomin |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yanlin |e verfasserin |4 aut | |
| 700 | 1 | |a Lang, Lixin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Shoujun |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Haitao |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Yanbao |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Xiaoyuan |e verfasserin |4 aut | |
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