Exosome-mediated aptamer S58 reduces fibrosis in a rat glaucoma filtration surgery model
International Journal of Ophthalmology Press..
AIM: To confirm whether exosome-mediated delivery of aptamer S58 (Exo-S58) has a better antifibrotic effect than naked S58 in human conjunctival fibroblasts (HConFs) and a rat glaucoma filtration surgery (GFS) model.
METHODS: To enhance the effective reaction time of aptamer S58 in vivo, we loaded aptamer S58 into exosomes derived from HEK293T cells by PEI transfection to determine the effect of Exo-S58 in HConFs and a rat GFS model.
RESULTS: Exo-S58 can significantly reduce cell proliferation, migration and fibrosis in TGF-β2-induced HConFs. In an in vivo experiment, Exo-S58 treatment prolonged filtering bleb retention and reduced fibrosis compared with naked S58 treatment in GFS rats.
CONCLUSION: The exosomes are safe and valid carriers to deliver aptamers. Furthermore, Exo-S58 exhibited superior antifibrotic effect than naked S58 both in HConFs cells and rat GFS models.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
International journal of ophthalmology - 15(2022), 5 vom: 05., Seite 690-700 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lin, Qian-Yi [VerfasserIn] |
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| Links: |
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| Themen: |
Anti-fibrosis |
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| Anmerkungen: |
Date Revised 16.07.2022 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.18240/ijo.2022.05.02 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM341237426 |
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| 245 | 1 | 0 | |a Exosome-mediated aptamer S58 reduces fibrosis in a rat glaucoma filtration surgery model |
| 264 | 1 | |c 2022 | |
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| 500 | |a Date Revised 16.07.2022 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a International Journal of Ophthalmology Press. | ||
| 520 | |a AIM: To confirm whether exosome-mediated delivery of aptamer S58 (Exo-S58) has a better antifibrotic effect than naked S58 in human conjunctival fibroblasts (HConFs) and a rat glaucoma filtration surgery (GFS) model | ||
| 520 | |a METHODS: To enhance the effective reaction time of aptamer S58 in vivo, we loaded aptamer S58 into exosomes derived from HEK293T cells by PEI transfection to determine the effect of Exo-S58 in HConFs and a rat GFS model | ||
| 520 | |a RESULTS: Exo-S58 can significantly reduce cell proliferation, migration and fibrosis in TGF-β2-induced HConFs. In an in vivo experiment, Exo-S58 treatment prolonged filtering bleb retention and reduced fibrosis compared with naked S58 treatment in GFS rats | ||
| 520 | |a CONCLUSION: The exosomes are safe and valid carriers to deliver aptamers. Furthermore, Exo-S58 exhibited superior antifibrotic effect than naked S58 both in HConFs cells and rat GFS models | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a anti-fibrosis | |
| 650 | 4 | |a aptamer | |
| 650 | 4 | |a drug delivery | |
| 650 | 4 | |a exosomes | |
| 650 | 4 | |a glaucoma surgery | |
| 650 | 4 | |a nanomedicine | |
| 650 | 4 | |a transforming growth factor-beta | |
| 700 | 1 | |a Li, Xiang-Ji |e verfasserin |4 aut | |
| 700 | 1 | |a Leng, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Xiao-Min |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Min |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Yi |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Wang-Du |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Bing-Cai |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Xia |e verfasserin |4 aut | |
| 700 | 1 | |a Xie, Lin |e verfasserin |4 aut | |
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| 773 | 1 | 8 | |g volume:15 |g year:2022 |g number:5 |g day:05 |g pages:690-700 |
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