Antibacterial, antioxidant, and cytotoxic activities of Syzygium aromaticum (L.) Merr. & Perry essential oil with identification of its chemical constituents
© 2022 Walter de Gruyter GmbH, Berlin/Boston..
Aromatic plants embrace volatile compounds with efficiency in treating different diseases. In Jordan, Syzygium aromaticum flower buds (clove) are extensively used as folk medicine without awareness of its bio-safe dosage. Herein, clove buds were hydrodistilled using the Clevenger apparatus, and the resulting essential oil (CEO) was analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). The antibacterial activity was evaluated against tested bacterial strains by agar diffusion test and micro-broth dilution assay. The antioxidant capacity was assessed using DPPH radical scavenging assay, while the cytotoxic potency was unraveled by determination of its anti-proliferative activity against MDA-MB-231 breast adenocarcinoma and normal Vero cell lines. CEO yield was 5.7 ± 1.3% (w/w); encompassed 24 volatile ingredients with eugenol as the principal compound (73.41%). The CEO inhibited the growth of both Gram-positive and Gram-negative bacterial test strains, causing the formation of 13.7 ± 1.5-17.3 ± 0.6 mm and 11.7 ± 1.5-20.7 ± 1.2 mm inhibition zones, respectively with MIC 1.25-5 μL/mL. Moreover, it showed antioxidant activity with IC50 0.0016 ± 0.0001 μL/mL (1.6 ± 0.1 μg/mL, 2.98 ± 0.4 µg Trolox®/µg CEO). Intriguingly, the CEO was cytotoxic against both cancerous and noncancerous cell lines at IC50 of 0.25 ± 0.02 μL/mL and 0.18 ± 0.01 μL/mL, respectively. Herein results unveil the potential application of CEO as a pharmaceutical remedy with considering its bio-safe dosage.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:78 |
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| Enthalten in: |
Zeitschrift fur Naturforschung. C, Journal of biosciences - 78(2023), 3-4 vom: 28. März, Seite 105-112 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Al-Zereini, Wael A [VerfasserIn] |
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| Links: |
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| Themen: |
Anti-Bacterial Agents |
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| Anmerkungen: |
Date Completed 10.03.2023 Date Revised 10.03.2023 published: Electronic-Print Citation Status MEDLINE |
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| doi: |
10.1515/znc-2022-0056 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM341218200 |
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| 100 | 1 | |a Al-Zereini, Wael A |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Antibacterial, antioxidant, and cytotoxic activities of Syzygium aromaticum (L.) Merr. & Perry essential oil with identification of its chemical constituents |
| 264 | 1 | |c 2023 | |
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| 500 | |a Date Revised 10.03.2023 | ||
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| 520 | |a © 2022 Walter de Gruyter GmbH, Berlin/Boston. | ||
| 520 | |a Aromatic plants embrace volatile compounds with efficiency in treating different diseases. In Jordan, Syzygium aromaticum flower buds (clove) are extensively used as folk medicine without awareness of its bio-safe dosage. Herein, clove buds were hydrodistilled using the Clevenger apparatus, and the resulting essential oil (CEO) was analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). The antibacterial activity was evaluated against tested bacterial strains by agar diffusion test and micro-broth dilution assay. The antioxidant capacity was assessed using DPPH radical scavenging assay, while the cytotoxic potency was unraveled by determination of its anti-proliferative activity against MDA-MB-231 breast adenocarcinoma and normal Vero cell lines. CEO yield was 5.7 ± 1.3% (w/w); encompassed 24 volatile ingredients with eugenol as the principal compound (73.41%). The CEO inhibited the growth of both Gram-positive and Gram-negative bacterial test strains, causing the formation of 13.7 ± 1.5-17.3 ± 0.6 mm and 11.7 ± 1.5-20.7 ± 1.2 mm inhibition zones, respectively with MIC 1.25-5 μL/mL. Moreover, it showed antioxidant activity with IC50 0.0016 ± 0.0001 μL/mL (1.6 ± 0.1 μg/mL, 2.98 ± 0.4 µg Trolox®/µg CEO). Intriguingly, the CEO was cytotoxic against both cancerous and noncancerous cell lines at IC50 of 0.25 ± 0.02 μL/mL and 0.18 ± 0.01 μL/mL, respectively. Herein results unveil the potential application of CEO as a pharmaceutical remedy with considering its bio-safe dosage | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Syzygium aromaticum | |
| 650 | 4 | |a antibacterial activity | |
| 650 | 4 | |a antioxidant capacity | |
| 650 | 4 | |a clove | |
| 650 | 4 | |a cytotoxicity | |
| 650 | 7 | |a Oils, Volatile |2 NLM | |
| 650 | 7 | |a clove flower extract |2 NLM | |
| 650 | 7 | |a Antioxidants |2 NLM | |
| 650 | 7 | |a Anti-Bacterial Agents |2 NLM | |
| 650 | 7 | |a Antineoplastic Agents |2 NLM | |
| 700 | 1 | |a Al-Trawneh, Ibrahim N |e verfasserin |4 aut | |
| 700 | 1 | |a Al-Qudah, Mahmoud A |e verfasserin |4 aut | |
| 700 | 1 | |a TumAllah, Heba M |e verfasserin |4 aut | |
| 700 | 1 | |a Abudayeh, Zead H |e verfasserin |4 aut | |
| 700 | 1 | |a Hijazin, Tahani |e verfasserin |4 aut | |
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| 856 | 4 | 0 | |u http://dx.doi.org/10.1515/znc-2022-0056 |3 Volltext |
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