Apoptotic vesicles activate autophagy in recipient cells to induce angiogenesis and dental pulp regeneration
Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved..
Extracellular vesicles (EVs) derived from living cells play important roles in donor cell-induced recipient tissue regeneration. Although numerous studies have found that cells undergo apoptosis after implantation in an ischemic-hypoxic environment, the roles played by the EVs released by apoptotic cells are largely unknown. In this study, we obtained apoptotic vesicles (apoVs) derived from human deciduous pulp stem cells and explored their effects on the dental pulp regeneration process. Our work showed that apoVs were ingested by endothelial cells (ECs) and elevated the expression of angiogenesis-related genes, leading to pulp revascularization and tissue regeneration. Furthermore, we found that, at the molecular level, apoV-carried mitochondrial Tu translation elongation factor was transported and regulated the angiogenic activation of ECs via the transcription factor EB-autophagy pathway. In a beagle model of dental pulp regeneration in situ, apoVs recruited endogenous ECs and facilitated the formation of dental-pulp-like tissue rich in blood vessels. These findings revealed the significance of apoptosis in tissue regeneration and demonstrated the potential of using apoVs to promote angiogenesis in clinical applications.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
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| Enthalten in: |
Molecular therapy : the journal of the American Society of Gene Therapy - 30(2022), 10 vom: 05. Okt., Seite 3193-3208 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Zihan [VerfasserIn] |
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| Links: |
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| Themen: |
Angiogenesis |
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| Anmerkungen: |
Date Completed 10.10.2022 Date Revised 06.10.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ymthe.2022.05.006 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 245 | 1 | 0 | |a Apoptotic vesicles activate autophagy in recipient cells to induce angiogenesis and dental pulp regeneration |
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| 520 | |a Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Extracellular vesicles (EVs) derived from living cells play important roles in donor cell-induced recipient tissue regeneration. Although numerous studies have found that cells undergo apoptosis after implantation in an ischemic-hypoxic environment, the roles played by the EVs released by apoptotic cells are largely unknown. In this study, we obtained apoptotic vesicles (apoVs) derived from human deciduous pulp stem cells and explored their effects on the dental pulp regeneration process. Our work showed that apoVs were ingested by endothelial cells (ECs) and elevated the expression of angiogenesis-related genes, leading to pulp revascularization and tissue regeneration. Furthermore, we found that, at the molecular level, apoV-carried mitochondrial Tu translation elongation factor was transported and regulated the angiogenic activation of ECs via the transcription factor EB-autophagy pathway. In a beagle model of dental pulp regeneration in situ, apoVs recruited endogenous ECs and facilitated the formation of dental-pulp-like tissue rich in blood vessels. These findings revealed the significance of apoptosis in tissue regeneration and demonstrated the potential of using apoVs to promote angiogenesis in clinical applications | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Wu, Meiling |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Siying |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xuemei |e verfasserin |4 aut | |
| 700 | 1 | |a Huan, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Ye, Qingyuan |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Xiaoxue |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Anqi |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Xiaoyao |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Xiaoshan |e verfasserin |4 aut | |
| 700 | 1 | |a Ding, Feng |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Haokun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Peisheng |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Shiyu |e verfasserin |4 aut | |
| 700 | 1 | |a Jin, Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Xuan, Kun |e verfasserin |4 aut | |
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