Expression of miR-106b-5p in children with primary immune thrombocytopenia and its correlation with T cells

OBJECTIVES: To study the expression level of plasma miR-106b-5p in primary immune thrombocytopenia (ITP) and its correlation with the levels of T helper 17 cell (Th17) and regulatory T cell (Treg) and the Th17/Treg ratio.

METHODS: A total of 79 children with ITP (ITP group) and 40 healthy children (control group) were selected as subjects. According to the treatment response, the 79 children with ITP were divided into three groups: complete response (n=40), partial response (n=18), and non-response (n=21). Quantitative real-time PCR was used to measure the expression level of miR-106b-5p. Flow cytometry was used to measure the frequencies of Th17 and Treg, and the Th17/Treg ratio was calculated. The correlation of the expression level of plasma miR-106b-5p with the frequencies of Th17 and Treg and the Th17/Treg ratio was analyzed.

RESULTS: Compared with the control group, the ITP group had significantly higher levels of miR-106b-5p, Th17, and Th17/Treg ratio (P<0.05) and a significantly lower level of Treg (P<0.05). After treatment, the ITP group had significant reductions in the levels of miR-106b-5p, Th17, and Th17/Treg ratio (P<0.05) and a significant increase in the level of Treg (P<0.05). Compared with the partial response and non-response groups, the complete response group had significantly lower levels of miR-106b-5p, Th17, and Th17/Treg ratio (P<0.05) and a significantly higher level of Treg (P<0.05). The correlation analysis showed that in the children with ITP, the expression level of plasma miR-106b-5p was positively correlated with the Th17 level and the Th17/Treg ratio (r=0.730 and 0.816 respectively; P<0.001) and was negatively correlated with the Treg level (r=-0.774, P<0.001).

CONCLUSIONS: A higher expression level of miR-106b-5p and Th17/Treg imbalance may be observed in children with ITP. The measurement of miR-106b-5p, Th17, Treg, and Th17/Treg ratio during treatment may be useful to the evaluation of treatment outcome in children with ITP.

Medienart:

E-Artikel

Erscheinungsjahr:

2022

Erschienen:

2022

Enthalten in:

Zur Gesamtaufnahme - volume:24

Enthalten in:

Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics - 24(2022), 4 vom: 15. Apr., Seite 411-416

Sprache:

Chinesisch

Weiterer Titel:

儿童原发免疫性血小板减少症miR-106b-5p的表达及其与T细胞的相关性研究

Beteiligte Personen:

Wang, Wen-Fang [VerfasserIn]
Wang, Xu-Song [VerfasserIn]
Tan, San-Yang [VerfasserIn]
Zhong, Lan-Lan [VerfasserIn]
Chen, Jiang [VerfasserIn]

Links:

Volltext

Themen:

Child
Journal Article
MIRN106 microRNA, human
MiR-106b-5p
MicroRNAs
Primary immune thrombocytopenia
Regulatory T cell
T helper 17 cell

Anmerkungen:

Date Completed 10.05.2022

Date Revised 31.05.2022

published: Print

Citation Status MEDLINE

doi:

10.7499/j.issn.1008-8830.2110139

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM340558792