Investigation of the adsorption behavior of BSA with tethered lipid layer-modified solid-state nanopores in a wide pH range
This journal is © The Royal Society of Chemistry..
Nanopore technology was introduced for the study of the dynamic interactions between bovine serum albumin (BSA) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) phospholipids based on a modified nanopore. The results reveal that the interaction mechanism between DOPE and BSA is affected by the pH of the subphase. Far above the BSA isoelectric point (pH > 7), a weaker hydrophobic interaction and stronger electrostatic repulsion exist between the DOPE and BSA molecules. At pH = 7, the BSA structure nearly does not change, and the interaction is weak. At pH 5 and pH 6, BSA is marginally affected by the adsorption interaction, and below pH 5, the DOPE film becomes disordered, so there is a strong repulsive force interaction between the BSA and DOPE.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2019 |
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Erschienen: |
2019 |
Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
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Enthalten in: |
RSC advances - 9(2019), 27 vom: 14. Mai, Seite 15431-15436 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Tian, Haibing [VerfasserIn] |
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Links: |
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Anmerkungen: |
Date Revised 16.07.2022 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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doi: |
10.1039/c9ra00698b |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM340433957 |
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520 | |a This journal is © The Royal Society of Chemistry. | ||
520 | |a Nanopore technology was introduced for the study of the dynamic interactions between bovine serum albumin (BSA) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) phospholipids based on a modified nanopore. The results reveal that the interaction mechanism between DOPE and BSA is affected by the pH of the subphase. Far above the BSA isoelectric point (pH > 7), a weaker hydrophobic interaction and stronger electrostatic repulsion exist between the DOPE and BSA molecules. At pH = 7, the BSA structure nearly does not change, and the interaction is weak. At pH 5 and pH 6, BSA is marginally affected by the adsorption interaction, and below pH 5, the DOPE film becomes disordered, so there is a strong repulsive force interaction between the BSA and DOPE | ||
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700 | 1 | |a He, Shixuan |e verfasserin |4 aut | |
700 | 1 | |a Zhou, Daming |e verfasserin |4 aut | |
700 | 1 | |a Fang, Shaoxi |e verfasserin |4 aut | |
700 | 1 | |a Liang, Liyuan |e verfasserin |4 aut | |
700 | 1 | |a Wang, Deqiang |e verfasserin |4 aut | |
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